Slater D N, Sloan J M
Atherosclerosis. 1975 Mar-Apr;21(2):259-72. doi: 10.1016/0021-9150(75)90085-4.
Endothelial cells from porcine aorta and inferior vena cava have been harvested, using trypsin, EDTA or collagenase, and grown in tissue culture. Growth-behaviour, cytology, scanning and electronmicroscopy findings are reported. It is hoped that this technique will prove useful in the investigation of atherosclerosis.
已使用胰蛋白酶、乙二胺四乙酸(EDTA)或胶原酶从猪主动脉和下腔静脉中获取内皮细胞,并在组织培养中进行培养。报告了其生长行为、细胞学、扫描电镜和透射电镜检查结果。希望该技术将被证明对动脉粥样硬化的研究有用。