Tokunaga O, Morimatsu M, Nakashima T
Br J Exp Pathol. 1984 Apr;65(2):165-70.
Variant endothelial cells were cultured from the healthy carotid arteries of a 19-year-old woman who was killed accidentally. The cells were grown and subcultured in Medium 199 supplemented with 20% heat-inactivated fetal calf serum. The cells are still viable after 20 passages. They were recognized as variant endothelial cells by their morphology which included ring formation, and by the existence of factor VIII-related surface antigen on the cell membrane. The cultured endothelial cells produced prostacyclin when the cells were incubated with arachidonic acid in smaller amounts than does typical endothelium, but far in excess of that produced by vascular smooth muscle cells.
从一名意外死亡的19岁女性健康颈动脉中培养出变异内皮细胞。这些细胞在添加20%热灭活胎牛血清的199培养基中生长并传代培养。传代20次后细胞仍存活。根据其形态（包括环状形成）以及细胞膜上存在VIII因子相关表面抗原，它们被识别为变异内皮细胞。当将培养的内皮细胞与花生四烯酸一起孵育时，其产生前列环素的量比典型内皮细胞少，但远远超过血管平滑肌细胞产生的量。
