痘苗病毒-gB重组体感染的细胞中人巨细胞病毒（HCMV）-gB蛋白的高表达：gB蛋白在HCMV免疫中的重要性。

High expression of human cytomegalovirus (HCMV)-gB protein in cells infected with a vaccinia-gB recombinant: the importance of the gB protein in HCMV immunity.

作者信息

Gönczöl E, deTaisne C, Hirka G, Berencsi K, Lin W C, Paoletti E, Plotkin S

机构信息

Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.

出版信息

Vaccine. 1991 Sep;9(9):631-7. doi: 10.1016/0264-410x(91)90187-b.

DOI:10.1016/0264-410x(91)90187-b

PMID:1659051

Abstract

The human cytomegalovirus (HCMV), Towne strain, glycoprotein B (gB) gene was cloned into a vaccinia vector (Copenhagen strain) under the control of the H6 early and late vaccinia promoters (Vac-gB recombinant). The gB protein was expressed in a high percentage of the Vac-gB-infected cells throughout the virus replication cycle. Cytosine-arabinoside (ara-C) did not influence the expression of the gB protein early after infection (5 h), but did inhibit it later in viral replication (7-29 h). The Vac-gB recombinant induced HCMV neutralizing antibodies in guinea-pigs. Cells infected with the Vac-gB recombinant absorbed 50-88% of neutralizing activity of human sera obtained from volunteers previously inoculated with the Towne or Toledo strains and from naturally seropositive individuals.

摘要

人巨细胞病毒（HCMV）Towne株糖蛋白B（gB）基因在H6痘苗早期和晚期启动子的控制下被克隆到痘苗载体（哥本哈根株）中（Vac-gB重组体）。在整个病毒复制周期中，gB蛋白在高比例的Vac-gB感染细胞中表达。阿糖胞苷（ara-C）在感染早期（5小时）不影响gB蛋白的表达，但在病毒复制后期（7 - 29小时）会抑制其表达。Vac-gB重组体在豚鼠中诱导出HCMV中和抗体。用Vac-gB重组体感染的细胞吸收了先前接种过Towne株或Toledo株的志愿者以及自然血清阳性个体的人血清中50 - 88%的中和活性。