Britt W J, Vugler L, Stephens E B
Department of Pediatrics, School of Medicine, University of Alabama, Birmingham 35294.
J Virol. 1988 Sep;62(9):3309-18. doi: 10.1128/JVI.62.9.3309-3318.1988.
The human cytomegalovirus (HCMV) envelope glycoprotein complex gp55-116 was expressed in both Escherichia coli and cells infected with a recombinant vaccinia virus. E. coli produced a single protein of Mr 100,000 which approximated the size of the nonglycosylated gp55-116 precursor found in HCMV-infected cells. Cells infected with the recombinant vaccinia virus contained three intracellular forms of Mr 160,000, 150,000, and 55,000 which were detected by a monoclonal antibody reactive with gp55. Comparison of the immunological properties of these recombinant proteins indicated that several of the HCMV gp55-116 monoclonal antibodies and sera from patients infected with HCMV reacted with the vaccinia virus-derived proteins whereas a more restricted group of monoclonal antibodies recognized the E. coli-produced protein. Immunization of mice with either E. coli or vaccinia virus recombinant HCMV gp55-116 resulted in production of virus-neutralizing antibodies. In contrast to the almost exclusive production of complement-dependent neutralizing antibodies following immunization with recombinant vaccinia virus, the E. coli-derived protein induced complement-independent neutralizing antibodies.
人巨细胞病毒（HCMV）包膜糖蛋白复合物gp55 - 116在大肠杆菌和感染重组痘苗病毒的细胞中均有表达。大肠杆菌产生一种分子量为100,000的单一蛋白质，其大小与在HCMV感染细胞中发现的未糖基化的gp55 - 116前体相近。感染重组痘苗病毒的细胞含有三种细胞内形式的蛋白质，分子量分别为160,000、150,000和55,000，可被与gp55反应的单克隆抗体检测到。对这些重组蛋白免疫特性的比较表明，几种HCMV gp55 - 116单克隆抗体以及来自感染HCMV患者的血清与痘苗病毒衍生的蛋白发生反应，而一组更具特异性的单克隆抗体识别大肠杆菌产生的蛋白。用大肠杆菌或痘苗病毒重组HCMV gp55 - 116免疫小鼠可产生病毒中和抗体。与用重组痘苗病毒免疫后几乎只产生补体依赖性中和抗体不同，大肠杆菌衍生的蛋白诱导产生非补体依赖性中和抗体。
