Kadouri A, Atsmon D, Edelman M
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2260-4. doi: 10.1073/pnas.72.6.2260.
Cucumber hypocotyl DNA in neutral CsCl distributes into a mainband comprising 59% of the total, and two large satellite bands which contribute 41% to the DNA pattern. Organelle enrichment studies show that the densities of mitochondrial and chloroplast DNA coincide with those of the satellite bands. At least 12-19% of total cucumber DNA is associated with the cytoplasmic organelles. These values, which are several times larger than those usually quoted for higher plants, are correlated with an unusually low amount of DNA per haploid nucleus in cucumber. Synthesis of the satellite DNAs, as well as mainband DNA, is appreciably stimulated in vivo by application of the plant hormone, gibberellin. Endogenous and hormone-enhanced synthesis of the satellite DNAs is proportionately greater in target tissue showing a high rate of organelle synthetic activity.
黄瓜下胚轴DNA在中性氯化铯中的分布形成一条占总量59%的主带，以及两条对DNA图谱贡献41%的大卫星带。细胞器富集研究表明，线粒体和叶绿体DNA的密度与卫星带的密度一致。黄瓜总DNA中至少12 - 19%与细胞质细胞器相关。这些数值比通常报道的高等植物数值大几倍，这与黄瓜单倍体细胞核中DNA含量异常低有关。施用植物激素赤霉素能显著刺激体内卫星DNA以及主带DNA的合成。在显示出高细胞器合成活性的靶组织中，卫星DNA的内源性合成和激素增强合成比例更大。
