Department of Botany, University of Georgia, Athens, Georgia 30602.
Plant Physiol. 1974 Mar;53(3):366-9. doi: 10.1104/pp.53.3.366.
Methods are described by which ribosomal DNA can be enriched in subcellular fractions of carrot and soybean. With both carrot and cucumber it was possible to obtain a distinct satellite DNA which contained the rDNA. Hybridization values greater than 0.49% were necessary before a satellite component was observed. Saturation hybridization values for soybean, carrot, and cucumber DNA were 0.2, 0.49, and 1.14%, respectively. These values were increased 1.6- and 2-fold in soybean and carrot, respectively, but enrichment was not obtained for cucumber.
方法描述了如何在胡萝卜和大豆的亚细胞部分中富集核糖体 DNA。对于胡萝卜和黄瓜，都有可能获得含有 rDNA 的明显卫星 DNA。在观察到卫星成分之前，杂交值必须大于 0.49%。大豆、胡萝卜和黄瓜 DNA 的饱和杂交值分别为 0.2、0.49 和 1.14%。在大豆和胡萝卜中，这些值分别增加了 1.6 倍和 2 倍，但在黄瓜中没有得到富集。
