Lewis M E, Lakshmanan J, Nagaiah K, Macdonnell P C, Guroff G
Section on Intermediary Metabolism, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):1021-3. doi: 10.1073/pnas.75.2.1021.
Intraventricular administration of nanogram quantities of nerve growth factor to adult rats results in a marked increase in the activity of ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) in the brain. The increase occurs in all major brain regions and the activity is maximal by 7.5 hr after administration. The enzyme response to nerve growth factor increases in magnitude during maturation; the relative increase in ornithine decarboxylase activity in adult animals is much greater than that in young. Neither insulin nor bovine growth hormone was able to increase ornithine decarboxylase activity to the same extent as did nerve growth factor. When brain was separated into neuronal- and glial-enriched fractions, induction of ornithine decarboxylase was found in both, but a greater increase was observed in the glial fraction.
向成年大鼠脑室内注射纳克量的神经生长因子,会导致其大脑中鸟氨酸脱羧酶(L-鸟氨酸羧基裂解酶,EC 4.1.1.17)的活性显著增加。这种增加发生在所有主要脑区,给药后7.5小时活性达到最大值。在成熟过程中,该酶对神经生长因子的反应幅度会增加;成年动物中鸟氨酸脱羧酶活性的相对增加比幼年动物大得多。胰岛素和牛生长激素都不能像神经生长因子那样将鸟氨酸脱羧酶活性提高到相同程度。当将大脑分离为富含神经元和胶质细胞的部分时,在两者中都发现了鸟氨酸脱羧酶的诱导,但在胶质细胞部分观察到了更大的增加。