MacDonnell P C, Nagaiah K, Lakshmanan J, Guroff G
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4681-4. doi: 10.1073/pnas.74.10.4681.
Nerve growth factor produces a rapid increase in the activity of ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) in superior cervical ganglia of young rats in vivo and in vitro. Maximum activity occurs 6-7 hr after the addition of nerve growth factor. The nerve growth factor-mediated increase in ornithine decarboxylase activity in vitro can be prevented by the addition of cycloheximide, actinomycin D, or antibody to nerve growth factor. A number of other agents were tested for their ability to increase ornithine decarboxylase were tested for their ability to increase ornithine decarboxylase activity in the ganglia; only nerve growth factor, and, to a slight extent, insulin were able to raise the activity of the enzyme. High concentrations of dibutyryl cyclic AMP (10 mM) were able to mimic the effect of nerve growth factor.
神经生长因子在体内和体外均可使幼鼠颈上神经节中的鸟氨酸脱羧酶（L-鸟氨酸羧基裂解酶，EC 4.1.1.17）的活性迅速升高。在添加神经生长因子后6-7小时出现最大活性。体外实验中，添加环己酰亚胺、放线菌素D或神经生长因子抗体可阻止神经生长因子介导的鸟氨酸脱羧酶活性增加。测试了许多其他试剂增加神经节中鸟氨酸脱羧酶活性的能力；只有神经生长因子以及在一定程度上胰岛素能够提高该酶的活性。高浓度的二丁酰环磷腺苷（10 mM）能够模拟神经生长因子的作用。
