Englert A, Leclerc M
Laboratoire de Chimie Générale I, Faculté des Sciences, Université Libre de Bruxelles, B-1050 Bruxelles, Belgium.
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1050-1. doi: 10.1073/pnas.75.3.1050.
The equations representing the decay kinetics of fluorescence of a donor luminophore in the presence of an acceptor in the same flexible molecule are discussed. The assumption that distances between luminophores attached to chain ends are not correlated with the relative orientations of luminophores is tested by theoretical calculations for the unperturbed chains Tyr-(Ala)(n)-Tyr with n equal to 4 and 9. It appears that for these chains, in which the conformation is dominated by entropic factors, the above assumption is satisfactory even for chains with a small number of repeating units (n = 4).
讨论了在同一柔性分子中存在受体时供体发光体荧光衰减动力学的方程。通过对n等于4和9的未受干扰的链Tyr-(Ala)(n)-Tyr进行理论计算,检验了连接在链端的发光体之间的距离与发光体的相对取向不相关的假设。结果表明,对于这些构象受熵因素主导的链,即使对于重复单元数量较少的链(n = 4),上述假设也是令人满意的。