短的 5'侧翼区含有保守序列，这对于家蚕丝氨酸 tRNA 基因的活性是必需的。

A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.

机构信息

Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, Oregon 97403.

出版信息

Proc Natl Acad Sci U S A. 1983 Jun;80(11):3416-20. doi: 10.1073/pnas.80.11.3416.

DOI:10.1073/pnas.80.11.3416

PMID:16593326

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC394054/

Abstract

Using partially deleted genes, we have identified an upstream control signal required for transcription of a Bombyx mori (silkworm) tRNA(2) (Ala) gene. The 5' boundary of this essential region lies between 34 and 11 nucleotides preceding the transcription initiation site. Sequences in the 5' half of the tRNA(2) (Ala) coding region are also important. Both regions contain oligonucleotides that are conserved among several Bombyx genes transcribed by RNA polymerase III, suggesting that these sequences may have a general control function.

摘要

利用部分缺失的基因，我们已经鉴定出一个上游控制信号，该信号对于转录家蚕 tRNA(2) (Ala) 基因是必需的。这个必需区域的 5' 边界位于转录起始位点前 34 到 11 个核苷酸处。tRNA(2) (Ala) 编码区的 5' 半区的序列也很重要。这两个区域都包含寡核苷酸，这些寡核苷酸在 RNA 聚合酶 III 转录的几个家蚕基因中是保守的，这表明这些序列可能具有一般的控制功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeb9/394054/5a272ef18524/pnas00637-0283-a.jpg

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短的 5'侧翼区含有保守序列，这对于家蚕丝氨酸 tRNA 基因的活性是必需的。

A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.

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