根癌农杆菌的Ti质粒编码一种细胞分裂素生物合成酶。
T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.
作者信息
Akiyoshi D E, Klee H, Amasino R M, Nester E W, Gordon M P
出版信息
Proc Natl Acad Sci U S A. 1984 Oct;81(19):5994-8. doi: 10.1073/pnas.81.19.5994.
Abstract
Phytohormone overproduction in crown gall tumors is due to the expression of several T-DNA genes. The data strongly suggest that the tmr gene (transcript 4) is responsible for cytokinin overproduction by encoding dimethylallyl-pyrophosphate:AMP dimethylallyltransferase (DMA transferase), an enzyme directly involved in cytokinin biosynthesis. Cell-free extracts of Escherichia coli strains containing the tmr gene from pTiA6NC had DMA transferase activity. No activity was present in the control strain containing only the plasmid vector. The cytokinins synthesized were isopentenyladenine, isopentenyladenosine, and isopentenyladenosine 5'-monophosphate. DMA transferase activity was also detected in cloned crown gall tumors incited by Agrobacterium tumefaciens wild-type A6NC and a tms mutant. Enzymatic activity in cell-free extracts of E. coli and tumors could be abolished by transposon insertion within the tmr gene.
摘要
冠瘿瘤中植物激素的过量产生是由于几个T-DNA基因的表达。数据有力地表明，tmr基因（转录本4）通过编码二甲基烯丙基焦磷酸：AMP二甲基烯丙基转移酶（DMA转移酶）导致细胞分裂素过量产生，该酶直接参与细胞分裂素的生物合成。含有来自pTiA6NC的tmr基因的大肠杆菌菌株的无细胞提取物具有DMA转移酶活性。仅含有质粒载体的对照菌株中没有活性。合成的细胞分裂素是异戊烯腺嘌呤、异戊烯腺苷和异戊烯腺苷5'-单磷酸。在由根癌土壤杆菌野生型A6NC和tms突变体诱发的克隆冠瘿瘤中也检测到了DMA转移酶活性。tmr基因内的转座子插入可消除大肠杆菌和肿瘤无细胞提取物中的酶活性。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1983 Jan;80(2):407-11. doi: 10.1073/pnas.80.2.407.
2
Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.在毛叶冠瘿碱型根瘤中鉴定 TL-DNA 转录本的功能
EMBO J. 1982;1(1):147-52. doi: 10.1002/j.1460-2075.1982.tb01138.x.
3
The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.在毛叶番荔枝冠瘿瘤的 TL-DNA 中，编码了 7 个明确的多聚腺苷酸化转录本。
EMBO J. 1982;1(1):139-46. doi: 10.1002/j.1460-2075.1982.tb01137.x.
4
The molecular genetics of crown gall tumorigenesis.冠瘿瘤形成的分子遗传学
Adv Genet. 1984;22:209-83. doi: 10.1016/s0065-2660(08)60041-3.
5
Chemical regulation of growth and organ formation in plant tissues cultured in vitro.体外培养植物组织中生长和器官形成的化学调控
Symp Soc Exp Biol. 1957;11:118-30.
6
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EMBO J. 1983;2(3):403-9. doi: 10.1002/j.1460-2075.1983.tb01437.x.
7
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Annu Rev Microbiol. 1981;35:531-65. doi: 10.1146/annurev.mi.35.100181.002531.
8
Genetic analysis of T-DNA transcripts in nopaline crown galls.胭脂碱冠瘿瘤中T-DNA转录本的遗传分析。
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9
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