Suppr超能文献

结合二价阳离子对菌紫质光循环中天冬氨酸去质子化的重要性。

Importance of bound divalent cations to the tyrosine deprotonation during the photocycle of bacteriorhodopsin.

机构信息

Department of Chemistry and Biochemistry, University of California, 405 Hilgard Avenue, Los Angeles, CA 90024.

出版信息

Proc Natl Acad Sci U S A. 1985 Jun;82(11):3662-4. doi: 10.1073/pnas.82.11.3662.

DOI:10.1073/pnas.82.11.3662
PMID:16593567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397846/
Abstract

The transient absorption changes occurring at 297 nm during the photocycles of the deionized and acidified bacteriorhodopsins (blue membranes) were studied. As opposed to what happens during the photocycle of the purple membrane, for the blue membranes only the fast absorption increase corresponding to trans-cis isomerization of the retinal chromophore is present; the slow rise attributed to the tyrosine deprotonation is not observed. The addition of different salts to the deionized membrane restores the original color and causes a tyrosine deprotonation during the photocycle. This suggests that the presence of cations is required for the deprotonation of tyrosine as it is for the deprotonation of the retinylidene Schiff base. These results are discussed in terms of the recently proposed cation model for the observed deprotonation processes in the photocycle of bacteriorhodopsin.

摘要

在去离子和酸化的菌紫质（蓝膜）的光循环过程中，研究了在 297nm 处发生的瞬态吸收变化。与紫膜光循环过程中发生的情况相反，对于蓝膜，只有对应于视黄醛发色团的反式-顺式异构化的快速吸收增加是存在的；而没有观察到归因于酪氨酸去质子化的缓慢上升。向去离子膜中添加不同的盐会恢复原始颜色，并在光循环过程中引起酪氨酸去质子化。这表明，阳离子的存在对于酪氨酸的去质子化是必需的，就像对于视黄醛亚胺 Schiff 碱的去质子化一样。这些结果是根据最近提出的阳离子模型来讨论的，该模型用于解释菌紫质光循环中观察到的去质子化过程。

相似文献

1
Importance of bound divalent cations to the tyrosine deprotonation during the photocycle of bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3662-4. doi: 10.1073/pnas.82.11.3662.
2
On the molecular mechanisms of the Schiff base deprotonation during the bacteriorhodopsin photocycle.
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8580-4. doi: 10.1073/pnas.83.22.8580.
3
Effect of genetic modification of tyrosine-185 on the proton pump and the blue-to-purple transition in bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1990 Jun;87(11):4103-7. doi: 10.1073/pnas.87.11.4103.
4
Redshift of the purple membrane absorption band and the deprotonation of tyrosine residues at high pH: Origin of the parallel photocycles of trans-bacteriorhodopsin.
Biophys J. 1991 Aug;60(2):475-90. doi: 10.1016/S0006-3495(91)82074-4.
5
Evidence for the involvement of more than one metal cation in the Schiff base deprotonation process during the photocycle of bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4094-8. doi: 10.1073/pnas.84.12.4094.
6
Photoreactions of bacteriorhodopsin at acid pH.
Biophys J. 1989 Dec;56(6):1143-51. doi: 10.1016/S0006-3495(89)82761-4.
7
Substitution of amino acids Asp-85, Asp-212, and Arg-82 in bacteriorhodopsin affects the proton release phase of the pump and the pK of the Schiff base.
Proc Natl Acad Sci U S A. 1990 Feb;87(3):1018-22. doi: 10.1073/pnas.87.3.1018.
8
Interaction between Asp-85 and the proton-releasing group in bacteriorhodopsin. A study of an O-like photocycle intermediate.
Biochemistry. 1997 Apr 8;36(14):4135-48. doi: 10.1021/bi962322e.
9
Effects of tyrosine-26 and tyrosine-64 nitration on the photoreactions of bacteriorhodopsin.
Biochemistry. 1985 Dec 17;24(26):7733-40. doi: 10.1021/bi00347a035.
10
Cation binding by bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1985 Jan;82(2):396-400. doi: 10.1073/pnas.82.2.396.

引用本文的文献

1
Nature of the individual Ca binding sites in Ca-regenerated bacteriorhodopsin.
Biophys J. 1992 May;61(5):1201-6. doi: 10.1016/S0006-3495(92)81929-X.
2
Evidence for the involvement of more than one metal cation in the Schiff base deprotonation process during the photocycle of bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4094-8. doi: 10.1073/pnas.84.12.4094.
3
On the molecular mechanisms of the Schiff base deprotonation during the bacteriorhodopsin photocycle.
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8580-4. doi: 10.1073/pnas.83.22.8580.
4
Comparison of the dynamics of the primary events of bacteriorhodopsin in its trimeric and monomeric states.
Biophys J. 2002 Sep;83(3):1557-66. doi: 10.1016/S0006-3495(02)73925-8.
5
Specific binding sites for cations in bacteriorhodopsin.
Biophys J. 2001 Aug;81(2):1155-62. doi: 10.1016/S0006-3495(01)75772-4.
6
Time-resolved Fourier transform infrared spectroscopy of the polarizable proton continua and the proton pump mechanism of bacteriorhodopsin.
Biophys J. 2001 Feb;80(2):961-71. doi: 10.1016/S0006-3495(01)76075-4.
7
Binding of calcium ions to bacteriorhodopsin.
Biophys J. 1999 Jun;76(6):3219-26. doi: 10.1016/S0006-3495(99)77473-4.
8
Cation binding sites on the projected structure of bacteriorhodopsin.
Biophys J. 1986 Aug;50(2):277-84. doi: 10.1016/S0006-3495(86)83461-0.
9
Deprotonation of lipid-depleted bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1988 Aug;85(16):5918-22. doi: 10.1073/pnas.85.16.5918.
10
Tryptophan fluorescence quenching as a monitor for the protein conformation changes occurring during the photocycle of bacteriorhodopsin under different perturbations.
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5815-9. doi: 10.1073/pnas.86.15.5815.

本文引用的文献

1
Effect of iodination of the purple membrane on the photocycle of bacteriorhodopsin.
Arch Biochem Biophys. 1981 Dec;212(2):589-601. doi: 10.1016/0003-9861(81)90402-1.
2
The role of tyrosine residues in the function of bacteriorhodopsin. Specific nitration of tyrosine 26.
Eur J Biochem. 1981 Apr;115(3):595-604. doi: 10.1111/j.1432-1033.1981.tb06244.x.
3
Light-dependent nitration of bacteriorhodopsin.
Arch Biochem Biophys. 1983 Nov;227(1):321-8. doi: 10.1016/0003-9861(83)90376-4.
4
On the protein (tyrosine)-chromophore (protonated Schiff base) coupling in bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7083-7. doi: 10.1073/pnas.81.22.7083.
5
Salt and pH-dependent changes of the purple membrane absorption spectrum.
Photochem Photobiol. 1984 Nov;40(5):641-6. doi: 10.1111/j.1751-1097.1984.tb05353.x.
6
Bacteriorhodopsin and related pigments of halobacteria.
Annu Rev Biochem. 1982;51:587-616. doi: 10.1146/annurev.bi.51.070182.003103.
7
Rhodopsin-like protein from the purple membrane of Halobacterium halobium.
Nat New Biol. 1971 Sep 29;233(39):149-52. doi: 10.1038/newbio233149a0.
8
Photoreceptor protein from the purple membrane of Halobacterium halobium. Molecular weight and retinal binding site.
Biochemistry. 1976 Feb 24;15(4):792-8. doi: 10.1021/bi00649a010.
9
Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium.
Biophys J. 1975 Sep;15(9):955-62. doi: 10.1016/S0006-3495(75)85875-9.
10
Analogies between respiration and a light-driven proton pump as sources of energy for active glutamate transport in Halobacterium holobium.
Arch Biochem Biophys. 1977 Jan 15;178(1):308-14. doi: 10.1016/0003-9861(77)90196-5.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验