Division of Biological Sciences, University of Missouri, Columbia, MO 65211.
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7363-6. doi: 10.1073/pnas.83.19.7363.
The genetic analysis of higher plant mitochondria has been limited by a scarcity of identified mutations with known progenitors. Correspondingly, few molecular studies have been directed at types of plant mitochondrial variation other than cytoplasmic male sterility. The maternally inherited nonchromosomal stripe (NCS) mutants of maize have profound deleterious effects on plant growth and yield. We report specific alterations in mitochondrial DNA (mtDNA) for two independent, phenotypically distinct NCS mutants. NCS2 plants have a distinctive 21-kilobase Xho I mtDNA band and very reduced amounts of DNA in an 8-kilobase band that is present in the progenitor. NCS3 plants have a distinctive 20-kilobase Xho I band and a reduction in a 16-kilobase band. Our studies confirm that the affected organelle in NCS plants is the mitochondrion. Because NCS-type plants appear with a certain frequency in a particular line (WF9), this line is a potential source of additional mutations for functional and molecular analyses of maize mitochondrial genes.
高等植物线粒体的遗传分析受到已知前体突变体稀缺的限制。相应地，除细胞质雄性不育外，很少有分子研究针对其他类型的植物线粒体变异。玉米的母系遗传非染色体条纹（NCS）突变体对植物生长和产量有深远的有害影响。我们报告了两个独立的、表型不同的 NCS 突变体的线粒体 DNA（mtDNA）的特定改变。NCS2 植物具有独特的 21kb Xho I mtDNA 带，以及在祖代中存在的 8kb 带中非常少的 DNA。NCS3 植物具有独特的 20kb Xho I 带和 16kb 带的减少。我们的研究证实，NCS 植物中受影响的细胞器是线粒体。由于 NCS 型植物在特定系（WF9）中以一定频率出现，因此该系是玉米线粒体基因功能和分子分析的其他突变的潜在来源。
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