Newton K J, Knudsen C, Gabay-Laughnan S, Laughnan J R
Division of Biological Sciences, University of Missouri, Columbia 65211.
Plant Cell. 1990 Feb;2(2):107-13. doi: 10.1105/tpc.2.2.107.
We describe a new maternally inherited maize mutation, nonchromosomal stripe 5 (NCS5), that adversely affects plant growth and yield. Mutant plants are characterized by reduced height, defective yellow striping on leaves, and aborted kernels on ears. NCS5 striped plants carry both normal and partially deleted versions of the mitochondrial cytochrome oxidase subunit 2 gene and exhibit greatly reduced levels of cox2 transcripts when compared with nonstriped control plants. Other mitochondrial genes and their mRNAs are not affected. Thus, the defective plant phenotype is correlated with a reduction in the number of functional cytochrome oxidase subunit 2 genes. The NCS5 mutant mitochondrial genome appears to have arisen by amplification of a rare homologous recombination product.
我们描述了一种新的母系遗传玉米突变体，非染色体条纹5（NCS5），它对植物生长和产量有不利影响。突变植株的特征是株高降低、叶片出现有缺陷的黄色条纹以及果穗上的籽粒败育。NCS5条纹植株携带线粒体细胞色素氧化酶亚基2基因的正常版本和部分缺失版本，与无条纹对照植株相比，其cox2转录本水平大幅降低。其他线粒体基因及其mRNA不受影响。因此，有缺陷的植物表型与功能性细胞色素氧化酶亚基2基因数量的减少相关。NCS5突变体线粒体基因组似乎是由一种罕见的同源重组产物扩增产生的。
