Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
EMBO J. 1987 Jun;6(6):1535-9. doi: 10.1002/j.1460-2075.1987.tb02397.x.
The genetic and molecular analyses of higher plant mitochondria can be facilitated by studying maternally-inherited mutations, such as the nonchromosomal stripe (NCS) mutants of maize, that have deleterious effects on plant growth. We have previously demonstrated a correlation between specific alterations in mitochondrial DNA and the expression of NCS phenotypes. In the present studies, the effects of the NCS2 mutation on mitochondrial gene expression are evaluated. Proteins synthesized by mitochondria isolated from NCS2 mutants and from related plants with normal growth have been compared. NCS2 mitochondria synthesize much reduced amounts of a single polypeptide. Probes corresponding to the mitochondrial DNA region altered in NCS2 hybridize to an aberrant set of transcripts in NCS2 mitochondria. Transcripts homologous to several previously characterized plant mitochondrial genes are similar in NCS2 and related non-mutant mitochondria.
高等植物线粒体的遗传和分子分析可以通过研究母系遗传突变来促进，例如玉米的非染色体条纹（NCS）突变体，这些突变体对植物生长有有害影响。我们之前已经证明了线粒体 DNA 的特定改变与 NCS 表型的表达之间存在相关性。在本研究中，评估了 NCS2 突变对线粒体基因表达的影响。比较了从 NCS2 突变体和相关生长正常的植物中分离的线粒体合成的蛋白质。NCS2 线粒体合成的单一多肽量大大减少。与 NCS2 中改变的线粒体 DNA 区域相对应的探针在 NCS2 线粒体中杂交到异常的转录本。与几种先前表征的植物线粒体基因同源的转录本在 NCS2 和相关非突变体线粒体中相似。
