Greenwald I, Coulson A, Sulston J, Priess J
Nucleic Acids Res. 1987 Mar 11;15(5):2295-307. doi: 10.1093/nar/15.5.2295.
We describe the assembly of a set of overlapping clones from the lin-12 III chromosomal region that spans approximately 600 kb, and the identification of two restriction fragment length polymorphisms, eP6 and eP7, that flank the lin-12 locus. A comparison of the physical map and the genetic map yields approximate measurements of 930 kb/map unit for the eP6--lin-12 interval and 830 kb/map unit for the lin-12--eP7 interval. We interpret these values as supporting the proposal that the apparent clustering of genes observed for C. elegans autosomes results from decreased recombination frequency in clusters and not from nonrandom distribution of genes on the physical map.
我们描述了来自lin-12Ⅲ染色体区域的一组重叠克隆的组装情况，该区域跨度约600 kb，并鉴定了位于lin-12基因座两侧的两个限制性片段长度多态性eP6和eP7。对物理图谱和遗传图谱的比较得出，eP6与lin-12区间的测量值约为930 kb/图谱单位，lin-12与eP7区间的测量值约为830 kb/图谱单位。我们认为这些值支持以下观点：秀丽隐杆线虫常染色体上观察到的基因明显聚集是由于基因簇中重组频率降低，而非基因在物理图谱上的非随机分布。
