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玉米醇脱氢酶基因（Adh1）的 cDNA 克隆和诱导。

cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize.

机构信息

Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, P.O. Box 1600, Canberra City, A.C.T. 2601, Australia.

出版信息

Proc Natl Acad Sci U S A. 1982 May;79(9):2981-5. doi: 10.1073/pnas.79.9.2981.

DOI:10.1073/pnas.79.9.2981
PMID:16593188
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346332/
Abstract

cDNA clones of Adh1, one of two genes encoding alcohol dehydrogenase (ADH; alcohol:NAD(+) oxidoreductase, EC 1.1.1.1) in the maize genome, have been isolated. They were derived from mRNA extracted from anaerobically treated roots of maize seedlings. Identification was initially made on the basis of molecular weight and electrophoretic properties of the in vitro polypeptide obtained in hybridization-release-translation experiments. The identification was confirmed by antibody precipitation and by the use of maize stocks having different genetic constitutions at the Adh1 locus. The sequence of the longest cDNA segment, approximately 900 base pairs, was determined and appears to code for 168 COOH-terminal amino acids and to have a 3' nontranslated region of 364 base pairs. Reverse Southern hybridizations established that two different Adh1-S stocks produce a mRNA of 1,650 nucleotides, whereas an additional mRNA of 1,750 nucleotides is produced in three Adh1-F stocks. A 50-fold increase in Adh1 mRNA level occurs during anaerobiosis, reaching a maximum at 5 hr. Return to aerobic conditions indicates a half-life of more than 18 hr for the anaerobically induced Adh1 mRNA.

摘要

已经分离到玉米基因组中编码醇脱氢酶（ADH；醇：NAD（+）氧化还原酶，EC 1.1.1.1）的两个基因之一 Adh1 的 cDNA 克隆。它们是从玉米幼苗厌氧处理的根中提取的 mRNA 衍生而来的。最初是根据体外杂交-释放-翻译实验中获得的多肽的分子量和电泳性质来鉴定的。鉴定通过抗体沉淀和使用在 Adh1 基因座具有不同遗传组成的玉米品系来确认。最长 cDNA 片段的序列约为 900 个碱基对，似乎编码 168 个 COOH 末端氨基酸，并且具有 3'非翻译区 364 个碱基对。反向 Southern 杂交表明，两种不同的 Adh1-S 品系产生 1650 个核苷酸的 mRNA，而三个 Adh1-F 品系则产生 1750 个核苷酸的额外 mRNA。在厌氧条件下，Adh1 mRNA 水平增加了 50 倍，在 5 小时达到最大值。返回有氧条件表明，厌氧诱导的 Adh1 mRNA 的半衰期超过 18 小时。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/a53e6647f8b5/pnas00448-0249-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/fad70ab19acf/pnas00448-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/a07946c1cf33/pnas00448-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/51dcef6f8f53/pnas00448-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/26e2b5139ca1/pnas00448-0249-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/351c6b9182fa/pnas00448-0249-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/a53e6647f8b5/pnas00448-0249-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/fad70ab19acf/pnas00448-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/a07946c1cf33/pnas00448-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/51dcef6f8f53/pnas00448-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/26e2b5139ca1/pnas00448-0249-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/351c6b9182fa/pnas00448-0249-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ddd/346332/a53e6647f8b5/pnas00448-0249-e.jpg

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Storage Protein Synthesis in Maize: Isolation of Zein-synthesizing Polyribosomes.玉米贮藏蛋白合成：醇溶蛋白合成多核糖体的分离。
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