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玉米醇脱氢酶 1 的两个等位基因具有 3'结构和多聚（a）添加多态性。

Two Alleles of Maize ALCOHOL DEHYDROGENASE 1 Have 3' Structural and Poly(a) Addition Polymorphisms.

机构信息

Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia.

出版信息

Genetics. 1986 Jun;113(2):449-67. doi: 10.1093/genetics/113.2.449.

DOI:10.1093/genetics/113.2.449
PMID:17246333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1202849/
Abstract

Two standard electrophoretic alleles of the maize alcohol dehydrogenase 1 locus (Adh1-1S and Adh1-1F) have been isolated and characterized. Restriction endonuclease mapping shows that a region of less than 5 kb is conserved in both alleles and is flanked both 5' and 3' by regions highly polymorphic for restriction sites. Nucleotide sequence comparison of these two alleles reveals that polymorphism in the 3' flanking region is due to rearrangements including tandem duplications, a transposable element-like insertion and a deletion. S1 nuclease analysis shows that both the Adh1-1S and the Adh1-1F alleles contain multiple poly(A) addition sites; four sites are observed for the Adh1-1S alleles and seven sites for the Adh1-1F allele. Only two of these poly(A) addition sites appear to be identical in the two alleles. No consensus signal for poly(A) addition is observed near any of these sites.

摘要

两个标准的玉米醇脱氢酶 1 座位（Adh1-1S 和 Adh1-1F）的电泳等位基因已被分离和鉴定。限制酶图谱表明，两个等位基因中都有不到 5kb 的区域保守，并且 5'和 3'两侧都有高度多态性的限制位点。这两个等位基因的核苷酸序列比较表明，3'侧翼区的多态性是由于串联重复、转座元件样插入和缺失等重排引起的。S1 核酸酶分析表明，Adh1-1S 和 Adh1-1F 等位基因都含有多个多聚（A）添加位点；Adh1-1S 等位基因观察到四个位点，Adh1-1F 等位基因观察到七个位点。这两个等位基因中只有两个多聚（A）添加位点似乎是相同的。在这些位点附近没有观察到多聚（A）添加的一致信号。

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本文引用的文献

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Genetics. 1983 Nov;105(3):733-43. doi: 10.1093/genetics/105.3.733.
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Purification of maize alcohol dehydrogenase-1 allozymes and comparison of their tryptic peptides.玉米乙醇脱氢酶-1同工酶的纯化及其胰蛋白酶肽段的比较。
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Nucleic Acids Res. 1984 May 11;12(9):3983-4000. doi: 10.1093/nar/12.9.3983.
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Tissue-specific expression of two mRNA species transcribed from a single vimentin gene.从单个波形蛋白基因转录而来的两种mRNA的组织特异性表达。
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A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.一对用于选择双酶切限制片段任一条DNA链的新型M13载体。
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