Department of Cellular and Developmental Biology, 16 Divinity Avenue, The Biological Laboratories, Harvard University, Cambridge, MA 02138.
Proc Natl Acad Sci U S A. 1988 Jan;85(2):391-5. doi: 10.1073/pnas.85.2.391.
The chloroplast gene psbA codes for the photosynthetic quinone-binding membrane protein Q(B), which is the target of the herbicide atrazine. This gene has been converted into a nuclear gene. The psbA gene from an atrazine-resistant biotype of Amaranthus hybridus has been modified by fusing its coding region to transcription-regulation and transit-peptide-encoding sequences of a bona fide nuclear gene. The constructs were introduced into the nuclear genome of tobacco by using the Agrobacterium tumor-inducing (Ti) plasmid system, and the protein product of nuclear psbA has been identified in the photosynthetic membranes of chloroplasts. Recovery of atrazine-tolerant transgenic plants shows that the product of the transplanted gene functions in photosynthesis. These experiments show that it is possible to modify chloroplast-gene-specified functions via nuclear-genome transformation and also raise evolutionary questions.
叶绿体基因 psbA 编码光合醌结合膜蛋白 Q(B)，该蛋白是除草剂莠去津的作用靶点。该基因已被转化为核基因。拟南芥抗莠去津生物型的 psbA 基因通过融合其编码区与一个真正的核基因的转录调控和转运肽编码序列而被修饰。通过利用农杆菌诱导（Ti）质粒系统将构建体导入烟草的核基因组中，并在叶绿体的光合膜中鉴定出核 psbA 的蛋白质产物。恢复对莠去津耐受的转基因植物表明，移植基因的产物在光合作用中起作用。这些实验表明，通过核基因组转化来修饰叶绿体基因指定的功能是可能的，同时也引发了进化方面的问题。
Zotero 插件
