Van den Broeck G, Timko M P, Kausch A P, Cashmore A R, Van Montagu M, Herrera-Estrella L
Nature. 1985;313(6001):358-63. doi: 10.1038/313358a0.
Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose 1,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.
可以构建嵌合基因,将叶绿体定位的核酮糖1,5-二磷酸羧化酶小亚基的转运肽与一种细菌蛋白融合。融合蛋白被转运到叶绿体中,并以与小亚基多肽前体类似的方式被切割。