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通过与1,5-二磷酸核酮糖羧化酶小亚基的转运肽融合将外源蛋白靶向导入叶绿体。

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.

作者信息

Van den Broeck G, Timko M P, Kausch A P, Cashmore A R, Van Montagu M, Herrera-Estrella L

出版信息

Nature. 1985;313(6001):358-63. doi: 10.1038/313358a0.

DOI:10.1038/313358a0
PMID:3969146
Abstract

Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose 1,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

摘要

可以构建嵌合基因，将叶绿体定位的核酮糖1,5-二磷酸羧化酶小亚基的转运肽与一种细菌蛋白融合。融合蛋白被转运到叶绿体中，并以与小亚基多肽前体类似的方式被切割。

相似文献

1
Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.通过与1,5-二磷酸核酮糖羧化酶小亚基的转运肽融合将外源蛋白靶向导入叶绿体。
Nature. 1985;313(6001):358-63. doi: 10.1038/313358a0.
2
Chloroplast transport of a ribulose bisphosphate carboxylase small subunit-5-enolpyruvyl 3-phosphoshikimate synthase chimeric protein requires part of the mature small subunit in addition to the transit peptide.核酮糖二磷酸羧化酶小亚基-5-烯醇丙酮酸-3-磷酸莽草酸合酶嵌合蛋白的叶绿体转运除了需要转运肽外，还需要部分成熟小亚基。
J Biol Chem. 1988 Oct 15;263(29):15104-9.
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Homologous and heterologous reconstitution of Golgi to chloroplast transport and protein import into the complex chloroplasts of Euglena.眼虫高尔基体到叶绿体运输及蛋白质导入复杂叶绿体的同源和异源重组
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Analysis of chloroplast transit peptide function using mutations in the carboxyl-terminal region.利用羧基末端区域的突变分析叶绿体转运肽的功能。
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Synthesis of the small subunit of ribulose-bisphosphate carboxylase from genes cloned into plasmids containing the SP6 promoter.从克隆到含有SP6启动子的质粒中的基因合成核酮糖-1,5-二磷酸羧化酶小亚基。
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A mammalian cytochrome fused to a chloroplast transit peptide is a functional haemoprotein and is imported into isolated chloroplasts.与叶绿体转运肽融合的哺乳动物细胞色素是一种功能性血红蛋白，并被导入分离的叶绿体中。
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An import-competent precursor of small subunit of ribulose-1,5-bisphosphate carboxylase generated by factor Xa cleavage from a beta-galactosidase fusion expressed in Escherichia coli.一种具有导入能力的1,5-二磷酸核酮糖羧化酶小亚基前体，由在大肠杆菌中表达的β-半乳糖苷酶融合蛋白经凝血因子Xa切割产生。
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Deletion of the carboxyl-terminal portion of the transit peptide affects processing but not import or assembly of the small subunit of ribulose-1,5-bisphosphate carboxylase.转运肽羧基末端部分的缺失会影响1,5-二磷酸核酮糖羧化酶小亚基的加工，但不影响其导入或组装。
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