Gough Richard E, Drury Sally E, Culver Francesca, Britton Paul, Cavanagh Dave
Avian Virology, VLA Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK.
Avian Pathol. 2006 Apr;35(2):122-6. doi: 10.1080/03079450600597733.
A virus (AV71/99) was isolated from a green-cheeked Amazon parrot by propagation and passage in both primary embryo liver cells derived from blue and yellow macaw (Ara ararauna) embryos and chicken embryo liver cells. Electron microscopic examination of cytopathic agents derived from both types of cell cultures suggested that it was a coronavirus. This was confirmed using a pan-coronavirus reverse transcriptase polymerase chain reaction that amplified part of gene 1 that encodes the RNA-dependent RNA polymerase. The deduced sequence of 66 amino acids had 66 to 74% amino acid identity with the corresponding sequence of coronaviruses in groups 1, 2 and 3. Several other oligonucleotide primer pairs that give PCR products corresponding to genes 3, 5, N and the 3'-untranslated region of infectious bronchitis virus, turkey coronavirus and pheasant coronavirus (all in group 3) failed to do so with RNA from the parrot coronavirus. This is the first demonstration of a coronavirus in a psittacine species.
通过在源自蓝黄金刚鹦鹉（Ara ararauna）胚胎的原代胚胎肝细胞和鸡胚肝细胞中传代培养，从一只绿颊亚马逊鹦鹉体内分离出一种病毒（AV71/99）。对两种细胞培养物中产生的细胞病变因子进行电子显微镜检查表明，它是一种冠状病毒。使用泛冠状病毒逆转录聚合酶链反应对此进行了确认，该反应扩增了编码RNA依赖性RNA聚合酶的基因1的部分序列。推导的66个氨基酸序列与第1、2和3组冠状病毒的相应序列具有66%至74%的氨基酸同一性。其他几对寡核苷酸引物可产生与传染性支气管炎病毒、火鸡冠状病毒和雉冠状病毒（均属于第3组）的基因3、5、N以及3'非翻译区相对应的PCR产物，但用鹦鹉冠状病毒的RNA进行检测时未能成功。这是首次在鹦鹉物种中证实冠状病毒的存在。
