Goldberg Jonathan M, Manning Gerard, Liu Allen, Fey Petra, Pilcher Karen E, Xu Yanji, Smith Janet L
Boston Biomedical Research Institute, Watertown, Massachusetts, United States of America.
PLoS Genet. 2006 Mar;2(3):e38. doi: 10.1371/journal.pgen.0020038. Epub 2006 Mar 31.
Dictyostelium discoideum is a widely studied model organism with both unicellular and multicellular forms in its developmental cycle. The Dictyostelium genome encodes 285 predicted protein kinases, similar to the count of the much more advanced Drosophila. It contains members of most kinase classes shared by fungi and metazoans, as well as many previously thought to be metazoan specific, indicating that they have been secondarily lost from the fungal lineage. This includes the entire tyrosine kinase-like (TKL) group, which is expanded in Dictyostelium and includes several novel receptor kinases. Dictyostelium lacks tyrosine kinase group kinases, and most tyrosine phosphorylation appears to be mediated by TKL kinases. About half of Dictyostelium kinases occur in subfamilies not present in yeast or metazoa, suggesting that protein kinases have played key roles in the adaptation of Dictyostelium to its habitat. This study offers insights into kinase evolution and provides a focus for signaling analysis in this system.
盘基网柄菌是一种在发育周期中具有单细胞和多细胞形式的广泛研究的模式生物。盘基网柄菌基因组编码285种预测的蛋白激酶，这与更为高等的果蝇的数量相似。它包含真菌和后生动物共有的大多数激酶类别的成员，以及许多以前被认为是后生动物特有的成员，这表明它们在真菌谱系中是次生丢失的。这包括整个类酪氨酸激酶（TKL）组，该组在盘基网柄菌中有所扩展，并且包括几种新型受体激酶。盘基网柄菌缺乏酪氨酸激酶组激酶，并且大多数酪氨酸磷酸化似乎由TKL激酶介导。盘基网柄菌约一半的激酶存在于酵母或后生动物中不存在的亚家族中，这表明蛋白激酶在盘基网柄菌适应其栖息地的过程中发挥了关键作用。这项研究为激酶进化提供了见解，并为该系统中的信号分析提供了重点。
