Sanada Yuichi, Yoshida Kazuhiro, Konishi Kazuo, Oeda Mamoru, Ohara Masahiro, Tsutani Yasuhiro
Department of Surgical Oncology, Research Institution for Radiation Biology and Medicine, Hiroshima University, Kasumi, Hiroshima, Japan.
Oncol Rep. 2006 May;15(5):1157-61.
The purpose of this study was to examine the expression pattern of MUC5AC and SOX2 in ampulla of vater adenocarcinoma and evaluate the association between expression of these gastric epithelial markers and the histologic phenotype of ampulla of vater carcinoma. Six surgically resected samples of ampulla of vater adenocarcinoma, including four intestinal type carcinomas and two pancreatobiliary type carcinomas, were studied. We performed immunohistochemistry with a monoclonal antibody against MUC5AC and a polyclonal anti-SOX2 antibody. In two of the four intestinal type carcinomas, MUC5AC and SOX2 were focally expressed in the superficial neoplastic mucosa. However, in the centre of the tumour and in other invasive lesions, including vascular invasive lesions and metastatic lymph nodes, neither MUC5AC nor SOX2 was expressed. In contrast, in both pancreatobiliary type carcinomas, expression of MUC5AC and SOX2 was maintained or increased in invasive lesions. Our immunohistochemistry data suggest that MUC5AC and SOX2 are associated with the pancreatobiliary phenotype of ampulla of vater carcinoma and involved in later events in carcinogenesis, such as invasion and metastasis.
本研究的目的是检测MUC5AC和SOX2在壶腹腺癌中的表达模式，并评估这些胃上皮标志物的表达与壶腹癌组织学表型之间的关联。研究了6例手术切除的壶腹腺癌样本，其中包括4例肠型癌和2例胰胆管型癌。我们使用抗MUC5AC单克隆抗体和抗SOX2多克隆抗体进行免疫组织化学检测。在4例肠型癌中的2例中，MUC5AC和SOX2在浅表肿瘤黏膜中呈局灶性表达。然而，在肿瘤中心以及其他浸润性病变（包括血管浸润性病变和转移淋巴结）中，MUC5AC和SOX2均未表达。相反，在2例胰胆管型癌中，MUC5AC和SOX2在浸润性病变中表达维持或增加。我们的免疫组织化学数据表明，MUC5AC和SOX2与壶腹癌的胰胆管表型相关，并参与致癌过程中的后期事件，如侵袭和转移。
