McKenzie J, King A, Hare J, Fulford T, Wilson B, Stanley M
Histopathology Department, Addenbrookes Hospital, Cambridge, U.K.
J Pathol. 1991 Sep;165(1):75-80. doi: 10.1002/path.1711650112.
A quantitative immunocytochemical study of large granular lymphocytes (LGLs) in the normal cervix and in human papillomavirus (HPV) associated disease was performed using a panel of monoclonal antibodies which included those for LGL surface markers CD56, CD16, and CD57. Only CD56-positive cells were found within the ectocervical epithelium and these cells increased in number in cervical intraepithelial neoplasia (CIN) in comparison with normal cervix. Examination of serial sections and double labelling suggests that these cells are CD3+, CD8+, CD56+, CD16+. The observed increase in number of this subset was not associated specifically with HPV infection but was related to CIN. Lymphocytes expressing all three LGL markers were found in the stroma and CD16(+)-positive cells clustered around endocervical glands with occasional cells extending into the endocervical epithelium. These results indicate that a small subset of LGLs which express T-cell markers is increased in number in CIN. Cells expressing classical NK markers are restricted to the stroma and are not found within the ectocervical epithelium.
利用一组单克隆抗体对正常宫颈及人乳头瘤病毒（HPV）相关疾病中的大颗粒淋巴细胞（LGL）进行了定量免疫细胞化学研究，这些抗体包括针对LGL表面标志物CD56、CD16和CD57的抗体。仅在外宫颈上皮内发现了CD56阳性细胞，与正常宫颈相比，这些细胞在宫颈上皮内瘤变（CIN）中的数量增加。连续切片检查和双重标记表明这些细胞为CD3 +、CD8 +、CD56 +、CD16 +。观察到的这一亚群数量增加并非与HPV感染特异性相关，而是与CIN有关。在基质中发现了表达所有三种LGL标志物的淋巴细胞，且CD16阳性细胞聚集在宫颈管腺周围，偶尔有细胞延伸至宫颈管上皮内。这些结果表明，表达T细胞标志物的一小部分LGL在CIN中的数量增加。表达经典自然杀伤细胞标志物的细胞局限于基质中，在外宫颈上皮内未发现。
