Sullivan V, Smith G L
Department of Pathology, University of Cambridge, U.K.
J Gen Virol. 1987 Oct;68 ( Pt 10):2587-98. doi: 10.1099/0022-1317-68-10-2587.
A recombinant vaccinia virus expressing herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) has been constructed. Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. wt. that were recognized by anti-HSV-1 sera. Rabbits and mice vaccinated with the live recombinant virus produced antibodies that recognized 48K, 57K and 61K mol. wt. proteins in HSV-1-infected cells. The gG polypeptides were present on cytoplasmic and nuclear membranes during infection with both HSV-1 and recombinant vaccinia virus gG-VAC. The 57K and 61K mol. wt. gG polypeptides were present in purified HSV-1 virions and were targets for antibody-mediated complement-dependent virus neutralization.
已构建出一种表达单纯疱疹病毒1型（HSV-1）糖蛋白G（gG）的重组痘苗病毒。感染这种重组病毒（gG-VAC）的细胞合成了分子量为48K、57K和61K的糖基化蛋白，这些蛋白能被抗HSV-1血清识别。用这种活重组病毒免疫的兔和小鼠产生了能识别HSV-1感染细胞中分子量为48K、57K和61K蛋白的抗体。在HSV-1和重组痘苗病毒gG-VAC感染期间，gG多肽存在于细胞质膜和核膜上。分子量为57K和61K的gG多肽存在于纯化的HSV-1病毒粒子中，并且是抗体介导的补体依赖性病毒中和作用的靶点。
