Welter M W, Welter C J, Chambers D M, Svensson L
Ambico, Inc., Dallas Center, Iowa.
Arch Virol. 1991;120(3-4):297-304. doi: 10.1007/BF01310485.
A porcine group C rotavirus (strain Cowden-AmC-1) was adapted and serially passaged in an established diploid swine testicular cell line (ST cells). Growth of group C rotavirus in ST cells, which in maintenance medium required trypsin, but not pancreatin resulted in cytopathic effect characterized by cellular stranding and subsequent cell lysis. Active replication and assembly were confirmed by RNA profile analysis, immune electron microscopy and immunofluorescence. Adaptation of non-group A rotavirus to a continuous cell line has not previously been reported and should facilitate progress in diagnostic procedures and vaccine development.
一株猪C组轮状病毒（考登 - AmC - 1株）在已建立的二倍体猪睾丸细胞系（ST细胞）中进行适应和连续传代。C组轮状病毒在ST细胞中生长，该细胞在维持培养基中需要胰蛋白酶而非胰淀粉酶，会导致细胞病变效应，其特征为细胞拉长并随后细胞裂解。通过RNA谱分析、免疫电子显微镜和免疫荧光证实了病毒的活跃复制和装配。此前尚未报道过非A组轮状病毒适应连续细胞系的情况，这应有助于诊断程序和疫苗开发的进展。
