Primus Thomas, Kohler Dennis J, Johnston John J, Sugihara Robert T, Pitt William C
USDA/APHIS/WS [corrected] National Wildlife Research Center, 4101 LaPorte Ave., Fort Collins, Colorado 80521, USA.
J Chromatogr Sci. 2006 Jan;44(1):1-5.
A reversed-phase ion-pair liquid chromatographic analysis combined with a solid-phase extraction clean-up method is used to assess the quantity of diphacinone residue found in invertebrates. Three invertebrate species are exposed to commercially available diphacinone-fortified bait used for rat control. The invertebrate samples are collected, frozen, and shipped to the laboratory. The samples are homogenized after cryogenic freezing. A portion of the homogenized samples are extracted with acidified chloroform-acetone, followed by cleanup with a silica solid-phase extraction column. Diphacinone is detected by UV absorption at 325 nm after separation by the chromatographic system. The method limit of detection (MLOD) for snail and slug samples averaged 0.055 and 0.066 mg/kg, respectively. Diphacinone residues in snail tissue ranges from 0.83 to 2.5 mg/kg for Oxychilus spp. The mean recoveries from snails at 0.20 and 2.0 are 97 +/- 21% and 84 +/- 6%. Diphacinone residues in slug tissue ranges from 1.3 to 4.0 mg/kg for Deroceras laeve and < MLOD to 1.8 mg/kg for Limax maximus, respectively. The mean recoveries from slugs at 0.20 and 2.0 mg/kg are 91% +/- 15% and 86% +/- 5%.
采用反相离子对液相色谱分析法结合固相萃取净化法来评估在无脊椎动物中发现的敌鼠残留量。三种无脊椎动物物种接触用于灭鼠的市售含敌鼠毒饵。收集无脊椎动物样本，冷冻后运往实验室。样本在低温冷冻后进行匀浆。匀浆后的部分样本用酸化氯仿 - 丙酮萃取，随后用硅胶固相萃取柱净化。通过色谱系统分离后，在325nm处通过紫外吸收检测敌鼠。蜗牛和蛞蝓样本的方法检测限（MLOD）平均分别为0.055和0.066mg/kg。对于尖口蜗牛属，蜗牛组织中的敌鼠残留量在0.83至2.5mg/kg之间。在0.20和2.0mg/kg添加水平下，从蜗牛中的平均回收率分别为97±21%和84±6%。对于光滑野蛞蝓，蛞蝓组织中的敌鼠残留量在1.3至4.0mg/kg之间，对于大蛞蝓，残留量在低于方法检测限至1.8mg/kg之间。在0.20和2.0mg/kg添加水平下，从蛞蝓中的平均回收率分别为91%±15%和86%±5%。
