一种用于植物表达的新型荧光 pH 探针。

A novel fluorescent pH probe for expression in plants.

机构信息

Zentrum für Biochemie und Molekularbiologie, Universität Kiel, Am Botanischen Garten 9, 24118 Kiel, Germany.

出版信息

Plant Methods. 2006 Apr 6;2:7. doi: 10.1186/1746-4811-2-7.

BACKGROUND

The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.

RESULTS

pHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.

CONCLUSION

Pt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.

背景

pH 值是控制活细胞中许多代谢和信号通路的重要参数。重组荧光 pH 指示剂（pHluorins）已成为监测细胞 pH 值的流行工具。它们源自最受欢迎的维多利亚多管发光水母 GFP（Av-GFP）。在这里，我们介绍了一种来自橙色海笔 Ptilosarcus gurneyi（Pt-GFP）的新型荧光 pH 报告蛋白，并将其性质与 pHluorins 进行了比较，以用于植物中的表达和使用。