水痘-带状疱疹病毒DNA的限制性内切酶分析

Restriction endonuclease analysis of varicella-zoster virus DNAs.

作者信息

Yamamoto S, Kabuta H, Shingu M

机构信息

Department of Virology, Kurume University School of Medicine, Japan.

出版信息

Kurume Med J. 1991;38(2):45-50. doi: 10.2739/kurumemedj.38.45.

DOI:10.2739/kurumemedj.38.45

PMID:1660090

Abstract

The DNAs of 14 strains of varicella-zoster virus (VZV) isolated from epidemiologically unrelated individuals were analyzed by restriction endonuclease cleavage and agarose gel electrophoresis. Small but distinct differences in restriction patterns between the isolates were observed when the DNAs were digested with each of four enzymes examined (EcoRI, SalI, PstI, and HindIII). Six out of 14 isolates (43%) were lacking the PstI cleavage site between K and N fragments; one isolate was lacking the HindIII cleavage site between M and N fragments. No changes were observed in restriction patterns in DNA of one of the strains after 33 passages in cultured cells.

摘要

对从流行病学上无关联个体中分离出的14株水痘 - 带状疱疹病毒（VZV）的DNA进行了限制性内切酶切割和琼脂糖凝胶电泳分析。当用所检测的四种酶（EcoRI、SalI、PstI和HindIII）中的每一种对DNA进行消化时，观察到分离株之间的限制性图谱存在微小但明显的差异。14株分离株中有6株（43%）在K和N片段之间缺少PstI切割位点；1株分离株在M和N片段之间缺少HindIII切割位点。其中一个菌株的DNA在培养细胞中传代33次后，其限制性图谱未观察到变化。