Kotyzová Dana, Eybl Vladislav, Mihaljevic Martin, Glattre Eystein
Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Medicine in Pilsen, Karlovarská 48, Pilsen, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Dec;149(2):329-33. doi: 10.5507/bp.2005.052.
The aim of this study was to evaluate the influence of arsenic and bromine exposure with or without iodine and selenium supplementation on the element level in the thyroid of rats. Four major groups of Wistar female rats were fed with respective diets: group A - standard diet, group B - iodine rich diet (10 mg I/kg food), group C - selenium rich diet (1 mg Se/kg) and group D - iodine and selenium rich diet (as in group B and C). Each group was divided into four subgroups per 7 animals each receiving either NaAsO(2) ip (6.5 mg.kg(-1) twice a week for two weeks and 3.25 mg.kg(-1) for six weeks) or KBr in drinking water (58.8 mg.l(-1)) for 8 weeks or combined administration of both substances. Remaining subgroup served as controls. After 8 weeks thyroid glands were analyzed by ICP-MS for As, Br, Se, and I content. The exposition of rat to arsenic or bromine causes the accumulation of these elements in the thyroid gland ( approximately 18 ppm of As, approximately 90 ppm of Br) and significantly affects iodine and selenium concentration in the thyroid. In iodine and/or selenium supplemented rats the bromine intake into the thyroid was lowered to approximately 50% of the level in unsupplemented animals. Also selenium thyroid level elevated due to KBr administration was lowered by iodine supplementation in the diet. The accumulation of arsenic in the thyroid was not influenced by selenium or iodine supplementation; however, As(III) administration increased iodine thyroid level and suppressed selenium thyroid level in selenium or iodine supplemented group of animals.
本研究的目的是评估砷和溴暴露（无论是否补充碘和硒）对大鼠甲状腺中元素水平的影响。将四组主要的Wistar雌性大鼠分别喂食相应的饮食：A组——标准饮食，B组——富含碘的饮食（10毫克碘/千克食物），C组——富含硒的饮食（1毫克硒/千克），D组——富含碘和硒的饮食（如B组和C组）。每组再分为四个亚组，每组7只动物，分别接受腹腔注射NaAsO₂（6.5毫克·千克⁻¹，每周两次，共两周，之后六周为3.25毫克·千克⁻¹）或饮用含KBr的水（58.8毫克/升）8周，或同时给予这两种物质。其余亚组作为对照。8周后，通过电感耦合等离子体质谱法（ICP-MS）分析甲状腺中的砷、溴、硒和碘含量。大鼠暴露于砷或溴会导致这些元素在甲状腺中积累（砷约为18 ppm，溴约为90 ppm），并显著影响甲状腺中的碘和硒浓度。在补充碘和/或硒的大鼠中，甲状腺对溴的摄入量降低至未补充动物水平的约50%。此外，饮食中补充碘会降低因给予KBr导致的甲状腺硒水平升高。甲状腺中砷的积累不受硒或碘补充的影响；然而，在补充硒或碘的动物组中，给予As(III)会提高甲状腺碘水平并抑制甲状腺硒水平。
