Fernandez-Durango R, Ramirez J M, Trivino A, Sanchez D, Paraiso P, Garcia De Lacoba M, Ramirez A, Salazar J J, Fernandez-Cruz A, Gutkowska J
Departamento de Medicina Interna III, Ocular Hospital Universitario de San Carlos, Ciudad Universitaria, Madrid, Spain.
Exp Eye Res. 1991 Nov;53(5):591-6. doi: 10.1016/0014-4835(91)90217-3.
ANF binding sites were analysed in the ciliary processes of rabbits with unilateral experimental glaucoma which had been induced by injecting alpha-chymotrypsin into the posterior chamber of the right eyes. The intraocular pressure (IOP) of glaucomatous eyes was significantly greater (28.4 +/- 4 mmHg) than that of normotensive control eyes (13.1 +/- 1.4 mmHg, mean +/- S.E.M., n = 23, P less than 0.05). ANF concentrations in aqueous humour and the ciliary processes were significantly higher in glaucomatous eyes (91 +/- 2 pg ml-1 and 30.4 +/- 4.2 pg g-1 wet weight) than in normal eyes (3.1 +/- 2.2 pg ml-1 and 10.2 +/- 2.7 pg g-1 wet weight, respectively, n = 6, P less than 0.01). The number of ANF-binding sites (Bmax) in the ciliary processes of glaucomatous rabbit eyes was significantly decreased in comparison to the controls (24 +/- 4 vs. 13 +/- 3 fmol mg-1 protein, n = 10, P less than 0.05). These data suggest that ANF receptors in the ciliary processes are down-regulated and that ANF may play an important role in the pathophysiology of experimental glaucoma.
对单侧实验性青光眼兔的睫状体中的心房钠尿肽（ANF）结合位点进行了分析。通过向右眼后房注射α-糜蛋白酶诱导青光眼。青光眼眼的眼内压（IOP）显著高于正常血压对照眼（分别为28.4±4 mmHg和13.1±1.4 mmHg，平均值±标准误，n = 23，P < 0.05）。青光眼眼中房水和睫状体中的ANF浓度显著高于正常眼（分别为91±2 pg/ml和30.4±4.2 pg/g湿重）（正常眼分别为3.1±2.2 pg/ml和10.2±2.7 pg/g湿重，n = 6，P < 0.01）。与对照组相比，青光眼兔眼睫状体中ANF结合位点的数量（Bmax）显著减少（分别为24±4和13±3 fmol/mg蛋白质，n = 10，P < 0.05）。这些数据表明，睫状体中的ANF受体下调，并且ANF可能在实验性青光眼的病理生理学中起重要作用。
