Ramis I, Roselló-Catafau J, Gómez G, Zabay J M, Fernández Cruz E, Gelpí E
Department of Neurochemistry, Centro de Investigación y Desarrollo, Barcelona, Spain.
J Chromatogr. 1991 Sep 20;557(1-2):507-13. doi: 10.1016/s0021-9673(01)87159-4.
Prostaglandin E2, thromboxane B2 and leukotriene B4 monocyte production have been determined in human immune deficiency virus (HIV)-infected drug users (n = 36) and healthy subjects (n = 29). Eicosanoids were extracted from the incubates using C18 solid-phase cartridges and determined by radioimmunoassay. An enhanced production of prostaglandin E2 and thromboxane B2 was detected in monocytes from HIV-positive drug users whether or not they had been previously stimulated with zymosan. Concomitant leukotriene B4 increases were not observed. The results reported in this paper indicate that altered cyclooxygenase arachidonic acid metabolism in monocytes from HIV-infected drug users is associated with the severe cellular immunodysfunction characteristic of AIDS. In contrast, leukotriene B4 does not seem to play a role in AIDS-associated immunosuppression.
已对36名感染人类免疫缺陷病毒（HIV）的吸毒者和29名健康受试者测定了前列腺素E2、血栓素B2和白三烯B4的单核细胞生成情况。使用C18固相柱从培养物中提取类花生酸，并通过放射免疫测定法进行测定。无论先前是否用酵母聚糖刺激过，在HIV阳性吸毒者的单核细胞中均检测到前列腺素E2和血栓素B2生成增强。未观察到白三烯B4相应增加。本文报道的结果表明，HIV感染吸毒者单核细胞中环氧合酶花生四烯酸代谢改变与艾滋病特有的严重细胞免疫功能障碍有关。相比之下，白三烯B4似乎在艾滋病相关的免疫抑制中不起作用。
