Frolkis V V, Shkapenko A L
Institute of Gerontology AMS USSR, Kiev.
Mech Ageing Dev. 1991 Sep;60(1):55-60. doi: 10.1016/0047-6374(91)90109-d.
The experiments on Wistar rats of different age-groups have shown that insulin (1.6 units/kg) provokes hyperpolarization of hepatocyte plasmatic membranes in adult rats, but not in old ones. The hyperpolarization can be prevented by actinomycin D and ouabain, while 2-aminopyridine is ineffective in this case. The appearance of hyperpolarization correlates with the activation of Na,K-ATPase in hepatocyte plasmatic membrane. It is shown that insulin injection provokes the appearance of Na,K-ATPase activation factor in the hepatocyte cytosol. The synthesis of this factor reduces with age.
对不同年龄组的Wistar大鼠进行的实验表明，胰岛素（1.6单位/千克）可使成年大鼠肝细胞质膜超极化，但对老年大鼠则无此作用。放线菌素D和哇巴因可阻止这种超极化，而2-氨基吡啶在这种情况下无效。超极化的出现与肝细胞质膜中钠钾ATP酶的激活相关。结果表明，注射胰岛素可促使肝细胞胞质溶胶中出现钠钾ATP酶激活因子。该因子的合成随年龄增长而减少。
