成年和老年大鼠肝脏微粒体单加氧酶诱导过程中质膜特性的变化。

Changes in the plasmatic membrane characteristics during microsomal monooxygenase induction in the liver of adult and old rats.

作者信息

Frolkis V V, Kobzar A L, Paramonova G I

机构信息

Institute of Gerontology, AMS of Ukraine, Kiev.

出版信息

Mech Ageing Dev. 1995 May 12;80(2):139-45. doi: 10.1016/0047-6374(94)01566-5.

DOI:10.1016/0047-6374(94)01566-5

PMID:7564564

Abstract

The experiments on adult (6-8 months) and old (24-26 months) male Wistar rats have shown that treatment of animals with phenobarbital results in a significant increase in hepatic microsomal enzyme content, plasmatic membrane Na+, K(+)-ATPase activities and the elevation of hepatocyte membrane potential value. It is presumed that the changes in plasmatic membrane characteristics during microsomal monooxygenase induction are related to the synthesis of specific intracellular factors (invertors). This assumption was verified by the experiments with 'cellular hybrid' system (cytosol--plasmatic membranes). Using this cross-systems, it was shown that the hepatocyte cytosol of rats treated with phenobarbital produced Na+, K(+)-ATPase activity. The extent of Na+, K(+)-ATPase activation was essentially lower when cytosol derived from old rat hepatocytes was used. The presence of specific factors that activated Na+, K(+)-ATPase in hepatocyte plasmatic membrane was also discovered in blood serum of induced adult and old rats.

摘要

对成年（6 - 8个月）和老年（24 - 26个月）雄性Wistar大鼠进行的实验表明，用苯巴比妥治疗动物会导致肝微粒体酶含量显著增加、质膜Na + 、K(+)-ATP酶活性增强以及肝细胞膜电位值升高。据推测，微粒体单加氧酶诱导过程中质膜特性的变化与特定细胞内因子（转化体）的合成有关。这一假设通过“细胞杂交”系统（胞质溶胶 - 质膜）实验得到了验证。利用这个交叉系统表明，用苯巴比妥治疗的大鼠肝细胞胞质溶胶产生了Na + 、K(+)-ATP酶活性。当使用来自老年大鼠肝细胞的胞质溶胶时，Na + 、K(+)-ATP酶的激活程度明显较低。在诱导的成年和老年大鼠的血清中也发现了激活肝细胞质膜中Na + 、K(+)-ATP酶的特定因子。