Frolkis V V, Kobzar A L, Tjukhtin G M
Institute of Gerontology, Kiev, Ukraine.
Mech Ageing Dev. 1992 Nov;66(2):187-94. doi: 10.1016/0047-6374(92)90135-z.
Experiments on adult (6-8-month-old and 26-28-month-old) Wistar rats revealed the hyperpolarization of plasmic membranes and activation of Na,K-ATPase of adrenocorticocytes in animals of both age groups and of hepatocytes of adult rats. No effect of testosterone was observed on the level of membrane potential and the activity of Na,K-ATPase of hepatocytes of old rats. The effect of testosterone was prevented by inhibitors of protein biosynthesis (actinomycin D and cycloheximide) and a specific inhibitor of Na,K-ATPase (ouabain), but not by K(+)-channel blocker 2-aminopyridine. Testosterone was assumed to synthesize the specific factor, capable of activating Na,K-ATPase of plasmic membranes. The cytosole of hepatocytes and the blood serum of adult testosterone-treated rats activated the Na,K-ATPase of isolated plasmic membranes of hepatocytes of adult and old intact rats. During aging there was a decrease in the capacity of cells to synthesize the specific factor, which activated Na,K-ATPase of plasmic membranes.
对成年（6 - 8月龄和26 - 28月龄）Wistar大鼠进行的实验表明，两个年龄组动物的肾上腺皮质细胞质膜发生超极化，成年大鼠肝细胞的钠钾ATP酶被激活。未观察到睾酮对老年大鼠肝细胞的膜电位水平和钠钾ATP酶活性有影响。蛋白质生物合成抑制剂（放线菌素D和环己酰亚胺）以及钠钾ATP酶特异性抑制剂（哇巴因）可阻止睾酮的作用，但钾离子通道阻滞剂2 - 氨基吡啶则不能。推测睾酮能合成一种能够激活质膜钠钾ATP酶的特异性因子。成年睾酮处理大鼠的肝细胞胞质溶胶和血清可激活成年和老年未处理大鼠肝细胞分离质膜的钠钾ATP酶。随着衰老，细胞合成激活质膜钠钾ATP酶的特异性因子的能力下降。
