Shibata D, Hansmann M L, Weiss L M, Nathwani B N
Department of Pathology, Los Angeles County-University of Southern California Medical Center.
Hum Pathol. 1991 Dec;22(12):1262-7. doi: 10.1016/0046-8177(91)90109-3.
Epstein-Barr viral (EBV) infections are associated with Hodgkin's disease (HD). To better characterize this relationship, fixed tissues of infectious mononucleosis, normal and reactive lymph nodes, lymph nodes with progressively transformed germinal centers, and biopsy specimens with the different subtypes of HD were analyzed by polymerase chain reaction (PCR). The presence or absence of EBV, the relative amounts of EBV, and the presence of multiple EBV genotypes as defined by amplification of a polymorphic EBV locus were determined for each specimen. Epstein-Barr virus could be detected from all specimens with infectious mononucleosis (eight of eight cases), generally in relatively large amounts, with multiple EBV genotypes evident in two cases. Epstein-Barr virus could not be detected from normal or reactive lymph nodes (none of 39 cases). Small amounts of EBV could be detected from a minority of cases with progressively transformed germinal centers (two of 16 cases), with multiple EBV genotypes evident in one case. Variable amounts of EBV could be detected from approximately half of the specimens with HD (26 of 50 cases). Epstein-Barr virus was most often detected in the subtypes of mixed cellularity (12 of 15 cases), nodular sclerosis (seven of 14 cases), and lymphocyte depletion (five of seven cases) compared with nodular lymphocyte predominance HD (two of 14 cases). In contrast to specimens with infectious mononucleosis and progressively transformed germinal centers, only one EBV genotype was evident in the specimens with HD. These findings are consistent with the hypothesis that some cases of HD may be directly associated with EBV.
爱泼斯坦-巴尔病毒（EBV）感染与霍奇金淋巴瘤（HD）相关。为了更好地描述这种关系，采用聚合酶链反应（PCR）分析了传染性单核细胞增多症、正常及反应性淋巴结、生发中心进行性转化的淋巴结以及不同亚型HD的活检标本的固定组织。确定每个标本中EBV的存在与否、EBV的相对量以及通过多态性EBV位点扩增定义的多种EBV基因型的存在情况。所有传染性单核细胞增多症标本（8例中的8例）均可检测到EBV，通常量相对较大，2例可见多种EBV基因型。正常或反应性淋巴结（39例中无一例）未检测到EBV。少数生发中心进行性转化的病例（16例中的2例）可检测到少量EBV，1例可见多种EBV基因型。约一半HD标本（50例中的26例）可检测到不同量的EBV。与结节性淋巴细胞为主型HD（14例中的2例）相比，EBV最常出现在混合细胞型（15例中的12例）、结节硬化型（14例中的7例）和淋巴细胞消减型（7例中的5例）亚型中。与传染性单核细胞增多症和生发中心进行性转化的标本不同，HD标本中仅可见一种EBV基因型。这些发现与某些HD病例可能与EBV直接相关的假说一致。
