Carbone A, Gloghini A, Zanette I, Canal B, Rizzo A, Volpe R
Division of Pathology, Istituto Nazionale di Ricovero e Cura a Carattere Scientifico, Aviano, Italy.
Virchows Arch A Pathol Anat Histopathol. 1993;422(1):39-45. doi: 10.1007/BF01605131.
The presence of Epstein-Barr virus (EBV) genome in Hodgkin's and Reed-Sternberg (HRS) cells, as detected using in situ hybridization (ISH) with biotinylated BamHI "V" probes, along with the expression of EBV-encoded latent membrane protein (LMP) and vimentin was examined in paraffin-embedded sections of 39 immunomorphologically characterized cases of Hodgkin's disease (HD). ISH demonstrated EBV in HRS cells in 15 of 39 cases, whereas LMP expression was detected in 11 of 39 cases, only in the presence of EBV genome detection. With the exception of 1 case, in which HRS cells expressed B-cell-associated antigens, the LMP-positive cases included specimens in which HRS cells were of non-B, non-T phenotype. LMP expression showed a stronger association with lymphocyte depletion (LD) (3/3) and mixed cellularity (MC) (6/11) than with lymphocyte predominance (0/5) or nodular sclerosis (2/20) subtypes. Vimentin expression on HRS cells was found in all the LMP-expressing cases and only in a fraction (13/28) of LMP-negative cases. This study supports the view that HD represents a heterogeneous group of diseases also in terms of EBV association, LMP expression being strongly related to the "aggressive" LD and MC histological subtypes. In light of the supposed interactions between vimentin and LMP, their co-expression on HRS cells, as detected in this study, provides further evidence for a significant role of EBV in the development of a proportion of HD cases.
采用生物素化的BamHI “V” 探针原位杂交（ISH）检测霍奇金病（HD）石蜡包埋切片中霍奇金和里德-斯腾伯格（HRS）细胞中EB病毒（EBV）基因组的存在情况，并检测EBV编码的潜伏膜蛋白（LMP）和波形蛋白的表达。ISH在39例经免疫形态学特征化的霍奇金病病例中，有15例在HRS细胞中检测到EBV，而LMP表达仅在检测到EBV基因组的39例中的11例中被检测到。除1例HRS细胞表达B细胞相关抗原的病例外，LMP阳性病例包括HRS细胞为非B、非T表型的标本。LMP表达与淋巴细胞消减型（LD）（3/3）和混合细胞型（MC）（6/11）的相关性比与淋巴细胞为主型（0/5）或结节硬化型（2/20）亚型更强。在所有表达LMP的病例以及仅部分（13/28）LMP阴性病例的HRS细胞中发现波形蛋白表达。本研究支持这样一种观点，即HD在EBV关联方面也代表一组异质性疾病，LMP表达与 “侵袭性” 的LD和MC组织学亚型密切相关。鉴于波形蛋白和LMP之间可能存在的相互作用，本研究中检测到它们在HRS细胞上的共表达，为EBV在一部分HD病例发展中的重要作用提供了进一步证据。
