Prestes-Carneiro Luiz Euribel, Freitas Selma de Bastos Zambeli de, Zago Sueli Cristina Schadeck, Miguel Nadia Araújo, Primo Oswaldo Bill, Iha Alberto Hiroshi, Espíndola Noeli Maria, Vaz Adelaide José
Departamento de Imunologia, Universidade do Oeste Paulista, 19050-900 Presidente Prudente, SP, Brazil.
Mem Inst Oswaldo Cruz. 2006 Feb;101(1):15-20. doi: 10.1590/s0074-02762006000100004. Epub 2006 Mar 30.
In order to evaluate the taeniosis-cysticercosis complex in a population of a peasants' settlement, located at Teodoro Sampaio, state of São Paulo, Brazil (longitude 52 degrees 36'12 ", latitude 22 degrees 17'12 ") a series of laboratory markers were determined. After signing an informed consent, participants answered a standardized questionnaire. To determine anti-Taenia solium cysticercus antibodies, the samples were tested by enzyme linked immunoabsorbent assay using 18-and 14-kDa antigen proteins from vesicular fluid of Taenia crassiceps (VF-Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Total IgE levels were determined by chemmiluminescence's assay and hemogram by flow cytometer flux counter. A total of 84 individuals, 5.9% presented anti-T. solium cysticercus antibodies in ELISA and 3.6% were strongly reactive in the 18/14 kDa immunoblotting confirmatory test. All of the individuals with positive antibodies showed elevated Total IgE levels. We conclude that the frequency of anti-T. solium cysticercus antibodies in this population is higher than other regions considered endemic in São Paulo. Thus, it is important to carry out surveys in Peasants' settlement areas with the objective of establishing public health measures for prevention and control of infectious diseases such as taeniosis-cysticercosis.
为了评估位于巴西圣保罗州特奥多罗·桑帕约（经度52度36分12秒，纬度22度17分12秒）一个农民定居点人群中的绦虫病-囊尾蚴病复合体，测定了一系列实验室指标。在签署知情同意书后，参与者回答了一份标准化问卷。为了检测抗猪带绦虫囊尾蚴抗体，使用来自肥胖带绦虫囊泡液的18 kDa和14 kDa抗原蛋白，通过酶联免疫吸附测定法对样本进行检测。对反应性和不确定的ELISA样本进行免疫印迹检测。通过化学发光法测定总IgE水平，通过流式细胞仪通量计数器测定血常规。共有84人，5.9%的人在ELISA中呈现抗猪带绦虫囊尾蚴抗体，3.6%的人在18/14 kDa免疫印迹确证试验中呈强反应性。所有抗体呈阳性的个体总IgE水平均升高。我们得出结论，该人群中抗猪带绦虫囊尾蚴抗体的频率高于圣保罗州其他被视为地方病流行区的地区。因此，重要的是在农民定居点地区开展调查，以便制定预防和控制绦虫病-囊尾蚴病等传染病的公共卫生措施。
