Universidade do Oeste Paulista, Department of Immunology, Presidente Prudente, SP, Brazil.
Pathog Glob Health. 2013 Mar;107(2):88-95. doi: 10.1179/2047773213Y.0000000079.
The goal of this study was to estimate the seroprevalence of Toxocara spp., Toxoplasma gondii, and Taenia solium metacestode infection and determine some of the associated risk factors for people living in the Dona Carmen settlement, Pontal of Paranapanema, São Paulo, Brazil.
Serum samples from 194 subjects were tested and participants answered a questionnaire. An enzyme-linked immunosorbent assay (ELISA) system based on Toxocara spp. excretory-secretory antigens obtained from the cultured second-stage larvae of Toxocara canis or vesicular fluid (VF) antigen from Taenia crassiceps metacestode was used to detect anti-Toxocara spp. IgG and IgE and anti-T. solium metacestode, respectively. For cysticercosis, the reactive ELISA samples were assayed by Western blotting using 18 kDa and 14 kDa proteins purified from VF. For T. gondii-specific IgG and IgM antibodies, anti-SAG-1, GRA-1, and GRA-7 epitope specificity was determined by ELISA.
Toxoplasma gondii IgG antibodies were found in 102/194 individuals (52·6%) with increased infections in females (P = 0·02) and those with ≤US$300 monthly income (P = 0·01). Positive IgM antibodies were detected in 21/194 individuals (10·8%). Antibodies specific to Toxocara spp. were found in 28/194 subjects (14·4%). All the individuals with Toxocara spp. also had T. gondii-specific IgG antibodies. Taenia solium metacestode antibodies were detected in 11 subjects (5·7%), but none were reactive based on Western blotting.
In spite of environmental, educational, and socioeconomic factors favoring parasite infection, the seropositivity rates of T. gondii, Toxocara spp., and T. solium metacestode-specific IgG antibodies are similar to the rates found in studies conducted in different populations in Brazil.
本研究旨在估计巴西圣保罗帕拉纳潘那马蓬塔尔多娜卡门定居点居民的旋毛虫属、刚地弓形虫和猪带绦虫囊尾蚴感染的血清流行率，并确定一些相关的危险因素。
检测了 194 名研究对象的血清样本，并让参与者回答了一份问卷。采用基于犬蛔虫培养的第二期幼虫的排泄分泌抗原或猪带绦虫囊尾蚴的囊液（VF）抗原的酶联免疫吸附试验（ELISA）系统，分别检测抗旋毛虫属 IgG 和 IgE 以及抗猪带绦虫囊尾蚴。对于囊虫病，通过 Western blot 检测反应性 ELISA 样本，使用 VF 中纯化的 18 kDa 和 14 kDa 蛋白作为探针。对于刚地弓形虫特异性 IgG 和 IgM 抗体，通过 ELISA 确定抗 SAG-1、GRA-1 和 GRA-7 表位的特异性。
在 194 名个体中，102/194 人（52.6%）发现了刚地弓形虫 IgG 抗体，女性感染者增多（P=0.02），月收入≤300 美元的感染者增多（P=0.01）。在 194 名个体中，21/194 人（10.8%）检测到阳性 IgM 抗体。在 194 名研究对象中，有 28 人（14.4%）发现了抗旋毛虫属抗体。所有抗旋毛虫属的个体也都有刚地弓形虫特异性 IgG 抗体。在 11 名个体（5.7%）中检测到猪带绦虫囊尾蚴抗体，但没有一个通过 Western blot 呈阳性。
尽管环境、教育和社会经济因素有利于寄生虫感染，但刚地弓形虫、旋毛虫属和猪带绦虫囊尾蚴特异性 IgG 抗体的血清阳性率与巴西不同人群的研究发现相似。
