Michaux-Charachon S, Bourg G, Jumas-Bilak E, Guigue-Talet P, Allardet-Servent A, O'Callaghan D, Ramuz M
Unité 431, Faculté de Médecine, Institut National de la Santé et de la Recherche Médicale, Nîmes, France.
J Bacteriol. 1997 May;179(10):3244-9. doi: 10.1128/jb.179.10.3244-3249.1997.
PacI and SpeI restriction maps were obtained for the two chromosomes of each of the six species of the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, and B. neotomae. Three complementary techniques were used: hybridization with the two replicons as probes, cross-hybridization of restriction fragments, and a new mapping method. For each type strain, a unique I-SceI site was introduced in each of the two replicons, and the location of SpeI sites was determined by linearization at the unique site, partial digestion, and end labeling of the fragments. The restriction and genetic maps of the six species were highly conserved. However, numerous small insertions or deletions, ranging from 1 to 34 kb, were observed by comparison with the map of the reference strain of the genus, B. melitensis 16M. A 21-kb Spel fragment specific to B. ovis was found in the small chromosome of this species. A 640-kb inversion was demonstrated in the B. abortus small chromosome. All of these data allowed the construction of a phylogenetic tree, which reflects the traditional phenetic classification of the genus.
获得了布鲁氏菌属六个物种中每个物种两条染色体的PacI和SpeI限制性酶切图谱：羊种布鲁氏菌、牛种布鲁氏菌、猪种布鲁氏菌、犬种布鲁氏菌、绵羊种布鲁氏菌和沙鼠种布鲁氏菌。使用了三种互补技术：以两个复制子为探针进行杂交、限制性片段的交叉杂交以及一种新的图谱绘制方法。对于每个模式菌株，在两个复制子中的每一个中引入一个独特的I-SceI位点，并通过在独特位点线性化、部分酶切以及片段的末端标记来确定SpeI位点的位置。六个物种的限制性酶切图谱和遗传图谱高度保守。然而，与该属参考菌株羊种布鲁氏菌16M的图谱相比，观察到许多小的插入或缺失，范围从1到34 kb。在绵羊种布鲁氏菌的小染色体中发现了一个21 kb的、该物种特有的Spel片段。在牛种布鲁氏菌的小染色体中证实存在一个640 kb的倒位。所有这些数据使得构建了一个系统发育树，该树反映了该属传统的表型分类。
