Yamamoto Mami, Ishii Akihiro, Nogi Yuichi, Inoue Akira, Ito Masahiro
REDS Group (Saitama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST), Saitama Small Enterprise Promotion Corporation, 3-12-18 Kamiaoki, Kawaguchi, Saitama 333-0844, Japan.
Extremophiles. 2006 Oct;10(5):421-6. doi: 10.1007/s00792-006-0516-1. Epub 2006 Apr 13.
Two novel denitrifying alkalithermophilic bacteria, AT-1 and AT-2, were isolated from manure-amended soil. The isolates grew at 35-65 degrees C with an optimum temperature at 50-60 degrees C, and pH 6.5-10.0 with an optimum pH at 9.5. Both isolates were Gram-positive, facultative anaerobic, non-motile rod-shaped bacteria. A phylogenetic analysis based on 16S rRNA sequence data indicated that both AT-1 and AT-2 are members of the genus Anoxybacillus. DNA-DNA hybridization revealed moderate relatedness between AT-1 and AT-2 and one phylogenetically related strain, A. pushchinensis K1 (69.5 and 69.1%, respectively). Comparative analysis of morphology and biochemical characteristics of the two isolates also showed similarity to A. pushchinensis K1. Based on these results, we identified AT-1 and AT-2 as A. pushchinensis. To our knowledge, this is the first report of denitrifying bacterium isolated from alkalithermophilic Anoxybacillus spp.
从施用过粪肥的土壤中分离出两株新型反硝化嗜碱嗜热细菌，AT-1和AT-2。分离菌株在35-65℃生长，最适温度为50-60℃，pH值为6.5-10.0，最适pH值为9.5。两株分离菌株均为革兰氏阳性、兼性厌氧、无运动性的杆状细菌。基于16S rRNA序列数据的系统发育分析表明，AT-1和AT-2均为嗜碱芽孢杆菌属的成员。DNA-DNA杂交显示AT-1和AT-2与一株系统发育相关菌株嗜碱芽孢杆菌K1之间具有中等相关性（分别为69.5%和69.1%）。对这两株分离菌株的形态和生化特征进行比较分析，也显示出与嗜碱芽孢杆菌K1相似。基于这些结果，我们将AT-1和AT-2鉴定为嗜碱芽孢杆菌。据我们所知，这是首次报道从嗜碱嗜热嗜碱芽孢杆菌属中分离出反硝化细菌。
