Xie X Sunney, Yu Ji, Yang Wei Yuan
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Science. 2006 Apr 14;312(5771):228-30. doi: 10.1126/science.1127566.
The combination of specific probes and advanced optical microscopy now allows quantitative probing of biochemical reactions in living cells. On selected systems, one can detect and track a particular protein with single-molecule sensitivity, nanometer spatial precision, and millisecond time resolution. Metabolites, usually difficult to detect, can be imaged and monitored in living cells with coherent anti-Stokes Raman scattering microscopy. Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism.
特定探针与先进光学显微镜的结合，如今使得对活细胞内生化反应进行定量探测成为可能。在选定的系统中，人们能够以单分子灵敏度、纳米级空间精度和毫秒级时间分辨率来检测和追踪特定蛋白质。代谢物通常难以检测，但利用相干反斯托克斯拉曼散射显微镜可在活细胞中对其进行成像和监测。在此，我们描述这些技术在研究基因表达、主动运输和脂质代谢中的应用。
