Noh Ji Hyun, Chung Kyung Nam, Kim Yong Bong
Department of Obstetrics and Gynecology, College of Medicine, Inje University, Paik Hospital, Chung-gu, Seoul, Korea.
J Korean Med Sci. 2006 Apr;21(2):304-8. doi: 10.3346/jkms.2006.21.2.304.
Heat shock proteins (HSP) have been identified as an important factor of a very complex and highly conserved cellular defense mechanism to preserve cell survival under adverse environmental conditions. HSP 60 are immunodominant antigens of microbe such as Chlamydia trachomatis and have a potentiality to become a target antigen due to antigenic similarity between chlamydial and human HSP. This study was conducted to investigate the effects of Vero cell coculture to anti-HSP 60 on the early mouse embryo development in vitro. The 2-cell mouse embryos (ICR) were cultured and mouse embryo development was observed every 24 hr for 3 days. 45% and 22.1% of the embryos cultured in Ham's F-10 plus anti HSP 60 with Vero cells developed to the 4- to 8- cell stage (day 1) and morular stage (day 2) as compared with 29.2% and 2.7% of those cultured without Vero cells respectively. But at day 3, the beneficial effect of Vero cells was not noted. These findings suggest that Vero cells have some roles to overcome the detrimental effect of anti-HSP 60 to some degree. These results suggest that Vero cells coculture will promote reproductive outcome in patient previously sensitized to microbial (e.g. Chlamydia trachomatis) HSP 60.
热休克蛋白（HSP）已被确认为一种非常复杂且高度保守的细胞防御机制的重要因素，该机制可在不利环境条件下维持细胞存活。HSP 60是沙眼衣原体等微生物的免疫显性抗原，由于衣原体和人类HSP之间的抗原相似性，它有潜力成为靶抗原。本研究旨在探讨Vero细胞共培养对体外早期小鼠胚胎发育过程中抗HSP 60的影响。将2细胞期小鼠胚胎（ICR）进行培养，每24小时观察一次小鼠胚胎发育情况，持续3天。与分别在无Vero细胞条件下培养的胚胎（29.2%和2.7%）相比，在含有抗HSP 60的Ham's F-10培养基中与Vero细胞共培养的胚胎，有45%和22.1%发育到4至8细胞期（第1天）和桑葚胚期（第2天）。但在第3天，未观察到Vero细胞的有益作用。这些发现表明，Vero细胞在一定程度上具有克服抗HSP 60有害作用的作用。这些结果表明，Vero细胞共培养将改善先前对微生物（如沙眼衣原体）HSP 60致敏患者的生殖结局。
