Kim Yun Seog, Kim Min Ju, Kim Pil, Kim Jung Hoe
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Guseong-dong, 373-1, Yusong-gu, Daejeon, 305-701, Korea.
Biotechnol Lett. 2006 Mar;28(5):357-62. doi: 10.1007/s10529-005-5935-z.
A gene encoding the antimicrobial peptide, lactococcin K, was isolated from Lactococcus lactis subsp. lactis MY23 then cloned and expressed in Escherichia coli. Because the expressed lactococcin K was formed as an inclusion body in recombinant E. coli, a fusion protein containing lactococcin K and maltose-binding protein (MBP) was produced in a soluble form. For high-level production of lactococcin K, we performed a pH-stat fed-batch culture to produce 43,000 AU lactococcin K ml(-1) in 12 h.
从乳酸乳球菌乳酸亚种MY23中分离出一种编码抗菌肽乳球菌素K的基因，然后将其克隆并在大肠杆菌中表达。由于表达的乳球菌素K在重组大肠杆菌中形成包涵体，因此制备了一种含有乳球菌素K和麦芽糖结合蛋白（MBP）的融合蛋白，其以可溶形式存在。为了高水平生产乳球菌素K，我们进行了pH值恒定补料分批培养，在12小时内生产出43,000 AU/ml的乳球菌素K。
