Merenbloom Samuel I, Koeniger Stormy L, Valentine Stephen J, Plasencia Manolo D, Clemmer David E
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Anal Chem. 2006 Apr 15;78(8):2802-9. doi: 10.1021/ac052208e.
Multidimensional ion mobility spectrometry (IMS-IMS and IMS-IMS-IMS) techniques have been combined with mass spectrometry (MS) and investigated as a means of generating and separating peptide and protein fragment ions. When fragments are generated inside a drift tube and then dispersed by IMS prior to MS analysis, it is possible to observe many features that are not apparent from MS analysis alone. The approach is demonstrated by examining fragmentation patterns arising from electrospray ion distributions of insulin chain B and ubiquitin. The multidimensional IMS approach makes it possible to select individual components for collisional activation and to disperse fragments based on differences in mobility prior to MS analysis. Such an approach makes it possible to observe many features not apparent by MS analysis alone.
多维离子迁移谱（IMS-IMS和IMS-IMS-IMS）技术已与质谱（MS）相结合，并作为一种产生和分离肽和蛋白质碎片离子的手段进行了研究。当碎片在漂移管内产生，然后在质谱分析之前通过离子迁移谱进行分散时，就有可能观察到许多仅通过质谱分析无法明显观察到的特征。通过研究胰岛素B链和泛素的电喷雾离子分布产生的碎片化模式，证明了该方法。多维离子迁移谱方法使得在质谱分析之前能够选择单个成分进行碰撞激活，并根据迁移率差异分散碎片。这种方法使得能够观察到许多仅通过质谱分析无法明显观察到的特征。
