Ateşşahin Ahmet, Türk Gaffari, Karahan Izzet, Yilmaz Seval, Ceribaşi Ali Osman, Bulmuş Ozgür
Department of Pharmacology and Toxicology, University of Firat, Elaziğ, Turkey.
Fertil Steril. 2006 Apr;85 Suppl 1:1216-22. doi: 10.1016/j.fertnstert.2005.11.035.
To investigate a possible protective role of lycopene on adriamycin (ADR)-induced spermiotoxicity using quantitative, biochemical and histopathological approaches.
Experimental study.
Firat University Medical School, Experimental Research Centre, Elazig, Turkey.
Twenty four Sprague Dawley rats (8-weeks old)
INTERVENTION(S): Adriamycin (10 mg kg(-1)) was intraperitoneally injected and lycopene (4 mg kg(-1)) was administered by gavage in corn oil.
MAIN OUTCOME MEASURE(S): Reproductive organ weights were evaluated along with epididymal sperm concentration, motility and morphology. Testicular histological findings, oxidative status and plasma testosterone levels were also determined.
RESULT(S): Lycopene ameliorated ADR-induced reductions in both testes and epididymis weights. ADR decreased sperm motility, increased total abnormal sperm rates, but epididymal sperm concentration was not changed compared to control. A marked normalization was achieved in sperm motility and morphology in pretreatment with lycopene. Although testosterone level was decreased in ADR group compared to control, no changes were observed in pretreatment group. An increase in malondialdehyde and a decrease reduced glutathione concentrations were detected in alone ADR group compared to control. Pretreatment with lycopene restored significantly malondialdehyde and reduced glutathione concentrations. ADR caused severe degenerative changes in germinative cells, atrophy in the diameter size of seminiferous tubules and germinative cell thickness. However, ADR-induced histopathological alterations were effectively reverted by pretreatment with lycopene.
CONCLUSION(S): This study clearly indicates that ADR treatment markedly impaired testicular function and that pretreatment with lycopene might prevent this toxicity.
采用定量、生化和组织病理学方法，研究番茄红素对阿霉素（ADR）诱导的精子毒性可能具有的保护作用。
实验研究。
土耳其埃拉泽省菲拉特大学医学院实验研究中心。
24只8周龄的斯普拉格·道利大鼠
腹腔注射阿霉素（10 mg kg⁻¹），并通过灌胃给予番茄红素（4 mg kg⁻¹），溶剂为玉米油。
评估生殖器官重量以及附睾精子浓度、活力和形态。还测定了睾丸组织学结果、氧化状态和血浆睾酮水平。
番茄红素改善了ADR诱导的睾丸和附睾重量减轻。ADR降低了精子活力，增加了总异常精子率，但与对照组相比，附睾精子浓度未发生变化。用番茄红素预处理后，精子活力和形态显著恢复正常。虽然与对照组相比，ADR组的睾酮水平降低，但预处理组未观察到变化。与对照组相比，单独ADR组检测到丙二醛增加，还原型谷胱甘肽浓度降低。用番茄红素预处理可显著恢复丙二醛和还原型谷胱甘肽浓度。ADR导致生精细胞发生严重退行性变化，曲细精管直径大小和生精细胞厚度萎缩。然而，用番茄红素预处理可有效逆转ADR诱导的组织病理学改变。
本研究清楚地表明，ADR治疗显著损害睾丸功能，而用番茄红素预处理可能预防这种毒性。
