Choi Deok Ho, Kang Dae Gill, Cui Xun, Cho Kyung Woo, Sohn Eun Jin, Kim Jin Sook, Lee Ho Sub
Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.
Life Sci. 2006 Aug 15;79(12):1178-85. doi: 10.1016/j.lfs.2006.03.019. Epub 2006 Mar 21.
The positive inotropic effect of the aqueous extract of Convallaria keiskei (ACK) and the possible mechanisms responsible for this effect were investigated in beating rabbit atria. ACK significantly increased atrial stroke volume, pulse pressure, and cAMP efflux in beating rabbit atria. The effects were not altered by pre-treatment with staurosporine and diltiazem, a non-selective protein kinase inhibitor and an L-type Ca2+ channel blocker, respectively. In addition, ACK markedly increased the K+ concentration in the beating atria-derived perfusate. Convallatoxin, a well-known digitalis-like cardiac glycosidic constituent of ACK, also increased atrial stroke volume and pulse pressure but did not alter the cAMP efflux level. The increases in atrial stroke volume and pulse pressure induced by convallatoxin were not also altered by pre-treatment with diltiazem. These results suggest that the ACK-induced positive inotropic effect in beating rabbit atria may, at least in part, be due to the digitalis-like activity of convallatoxin.
在搏动的兔心房中研究了铃兰水提取物（ACK）的正性肌力作用及其可能的作用机制。ACK显著增加了搏动兔心房的每搏输出量、脉压和cAMP流出。分别用非选择性蛋白激酶抑制剂星形孢菌素和L型Ca2+通道阻滞剂地尔硫卓预处理后，这些作用并未改变。此外，ACK显著增加了搏动心房灌流液中的K+浓度。铃兰毒苷是ACK中一种著名的类洋地黄强心苷成分，它也增加了心房每搏输出量和脉压，但未改变cAMP流出水平。地尔硫卓预处理也未改变铃兰毒苷引起的心房每搏输出量和脉压增加。这些结果表明，ACK在搏动兔心房中诱导的正性肌力作用可能至少部分归因于铃兰毒苷的类洋地黄活性。
