Claude Sivagami-Jean, Raman Gurusamy, Park Seon-Joo
Department of Life Sciences, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of Korea.
Plants (Basel). 2023 Jul 28;12(15):2797. doi: 10.3390/plants12152797.
The 'Lilly of the Valley' species, , is renowned for its fragrant white flowers and distinctive fresh and green floral scent, attributed to a rich composition of volatile organic compounds (VOCs). However, the molecular mechanisms underlying the biosynthesis of this floral scent remain poorly understood due to a lack of transcriptomic data. In this study, we conducted the first comparative transcriptome analysis of . , encompassing the leaf, flower, and root tissues. Our aim was to investigate the terpene synthase (TPS) genes and differential gene expression (DEG) patterns associated with essential oil biosynthesis. Through de novo assembly, we generated a substantial number of unigenes, with the highest count in the root (146,550), followed by the flower (116,434) and the leaf (72,044). Among the identified unigenes, we focused on fifteen putative genes, which are involved in the synthesis of mono- and sesquiterpenes, the key aromatic compounds responsible for the essential oil biosynthesis in . . The expression of these genes was validated using quantitative PCR analysis. Both DEG and qPCR analyses revealed the presence of genes in the flower transcriptome, responsible for the synthesis of various compounds such as geraniol, germacrene, kaurene, linalool, nerolidol, trans-ocimene and valencene. The leaf transcriptome exhibited genes related to the biosynthesis of kaurene and trans-ocimene. In the root, the identified unigenes were associated with synthesizing kaurene, trans-ocimene and valencene. Both analyses indicated that the genes involved in mono- and sesquiterpene biosynthesis are more highly expressed in the flower compared to the leaf and root. This comprehensive study provides valuable resources for future investigations aiming to unravel the essential oil-biosynthesis-related genes in the genus.
铃兰属植物以其芬芳的白色花朵以及独特的清新绿色花香而闻名，这种花香归因于丰富的挥发性有机化合物（VOCs）成分。然而，由于缺乏转录组数据，这种花香生物合成的分子机制仍知之甚少。在本研究中，我们首次对铃兰属植物进行了比较转录组分析，涵盖叶、花和根组织。我们的目的是研究与精油生物合成相关的萜烯合酶（TPS）基因和差异基因表达（DEG）模式。通过从头组装，我们生成了大量单基因，其中根中的数量最多（146,550个），其次是花（116,434个）和叶（72,044个）。在鉴定出的单基因中，我们重点关注了15个推定的TPS基因，它们参与单萜和倍半萜的合成，这些是铃兰属植物精油生物合成中关键的芳香化合物。使用定量PCR分析验证了这些基因的表达。DEG分析和qPCR分析均显示花转录组中存在TPS基因，这些基因负责合成各种化合物，如香叶醇、杜松烯、贝壳杉烯、芳樟醇、橙花叔醇、反式罗勒烯和瓦伦烯。叶转录组显示出与贝壳杉烯和反式罗勒烯生物合成相关的基因。在根中，鉴定出的单基因与贝壳杉烯、反式罗勒烯和瓦伦烯的合成有关。两项分析均表明，与叶和根相比，参与单萜和倍半萜生物合成的基因在花中的表达更高。这项全面的研究为未来旨在揭示铃兰属植物中与精油生物合成相关基因的研究提供了宝贵的资源。
