Dasgupta Amitava, Bourgeois Laverne
Department of Pathology and Laboratory Medicine, University of Texas McGovern Medical School at Houston, Houston, TX, USA.
Laboratory Services, Memorial-Hermann Hospital at Texas Medical Center, Houston, TX, USA.
J Clin Lab Anal. 2018 Oct;32(8):e22583. doi: 10.1002/jcla.22583. Epub 2018 May 31.
Lily of the valley is a poisonous plant due to the presence of the cardiac glycoside convallatoxin which is known to interfere with serum digoxin measurement using the LOCI digoxin assay and other digoxin assays. We evaluated potential interference of convallatoxin as well as extract of lily of the valley with the ADVIA Centaur digoxin assay by comparing results obtained using the LOCI digoxin assay.
Aliquots of a drug-free serum pool and a digoxin serum pool were supplemented with nanograms to 1 μg quantities of convallatoxin or 1.0 and 2.5 μL of lily of the valley extract per milliliter of serum followed by measurement of digoxin concentrations using the LOCI and ADVIA Centaur digoxin assays.
Apparent digoxin concentrations were minimal using the ADVIA Centaur digoxin assay when aliquots of drug-free serum were supplemented with convallatoxin or extract of lily of the valley but apparent digoxin levels were very high using the LOCI digoxin assay. Moreover, minimal interference in serum digoxin measurement using the ADVIA Centaur digoxin assay was observed when aliquots of serum digoxin pool were further supplemented with lily of the valley extract. As expected, the LOCI digoxin assay showed significant interference of convallatoxin in serum digoxin measurement.
Significant interference of convallatoxin in serum digoxin measurement using the LOCI digoxin assay could be minimized using the ADVIA Centaur digoxin assay.
铃兰是一种有毒植物，因其含有强心苷铃兰毒苷，已知该物质会干扰采用微粒体荧光免疫分析技术（LOCI）地高辛检测法及其他地高辛检测法进行的血清地高辛测定。我们通过比较使用LOCI地高辛检测法获得的结果，评估了铃兰毒苷以及铃兰提取物对ADVIA Centaur地高辛检测法的潜在干扰。
向不含药物的血清混合液和地高辛血清混合液的等分试样中，每毫升血清补充纳克至1微克量的铃兰毒苷或1.0和2.5微升铃兰提取物，然后使用LOCI和ADVIA Centaur地高辛检测法测量地高辛浓度。
当向不含药物的血清等分试样中补充铃兰毒苷或铃兰提取物时，使用ADVIA Centaur地高辛检测法测得的表观地高辛浓度最低，但使用LOCI地高辛检测法测得的表观地高辛水平非常高。此外，当地高辛血清混合液的等分试样进一步补充铃兰提取物时，观察到使用ADVIA Centaur地高辛检测法对血清地高辛测定的干扰最小。正如预期的那样，LOCI地高辛检测法显示铃兰毒苷在血清地高辛测定中存在显著干扰。
使用ADVIA Centaur地高辛检测法可将铃兰毒苷在使用LOCI地高辛检测法进行血清地高辛测定中的显著干扰降至最低。
