van der Vaart J M, Pant N, Wolvers D, Bezemer S, Hermans P W, Bellamy K, Sarker S A, van der Logt C P E, Svensson L, Verrips C T, Hammarstrom L, van Klinken B J W
Unilever R&D Vlaardingen, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands.
Vaccine. 2006 May 8;24(19):4130-7. doi: 10.1016/j.vaccine.2006.02.045. Epub 2006 Mar 7.
Apart from the use of oral rehydration solution, there are currently no treatment modalities for rotavirus induced diarrhoea, which is particularly relevant to developing countries. Fragments derived from llama heavy chain antibodies were previously shown to be highly stable, efficiently produced in yeast and exhibiting high epitope specific affinity. We now aim to demonstrate that these antibody fragments are capable of reducing morbidity of rotavirus induced diarrhoea. Here we show the isolation of rotavirus specific antibody fragments and their capability of reducing the morbidity of rotavirus induced diarrhoea in vivo in mice. They could provide a treatment modality for the moderation of human rotavirus infections having a significant impact on the course of an often fatal childhood disease.
除了使用口服补液盐外，目前对于轮状病毒引起的腹泻尚无治疗方法，这在发展中国家尤为重要。先前已证明，源自骆驼重链抗体的片段高度稳定，能在酵母中高效产生并表现出高表位特异性亲和力。我们现在旨在证明这些抗体片段能够降低轮状病毒引起的腹泻的发病率。在此我们展示了轮状病毒特异性抗体片段的分离及其在小鼠体内降低轮状病毒引起的腹泻发病率的能力。它们可为缓解人类轮状病毒感染提供一种治疗方法，这种感染对一种往往致命的儿童疾病的病程有重大影响。
